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Molecular and Cellular Biology, November 2004, p. 9568-9579, Vol. 24, No. 21
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.21.9568-9579.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Coordinated Temporal Interplay of Nucleosome Reorganization Factor, Sister Chromatin Cohesion Factor, and DNA Polymerase {alpha} Facilitates DNA Replication

Yanjiao Zhou and Teresa S.-F. Wang*

Department of Pathology, Stanford University School of Medicine, Stanford, California

Received 7 April 2004/ Returned for modification 14 May 2004/ Accepted 4 August 2004

DNA replication depends critically upon chromatin structure. Little is known about how the replication complex overcomes the nucleosome packages in chromatin during DNA replication. To address this question, we investigate factors that interact in vivo with the principal initiation DNA polymerase, DNA polymerase {alpha} (Pol{alpha}). The catalytic subunit of budding yeast Pol{alpha} (Pol1p) has been shown to associate in vitro with the Spt16p-Pob3p complex, a component of the nucleosome reorganization system required for both replication and transcription, and with a sister chromatid cohesion factor, Ctf4p. Here, we show that an N-terminal region of Pol{alpha} (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo. A mutation in a glycine residue in this N-terminal region of POL1 compromises the ability of Pol1p to associate with Spt16p and alters the temporal ordered association of Ctf4p with Pol1p. The compromised association between the chromatin-reorganizing factor Spt16p and the initiating DNA polymerase Pol1p delays the Pol1p assembling onto and disassembling from the late-replicating origins and causes a slowdown of S-phase progression. Our results thus suggest that a coordinated temporal and spatial interplay between the conserved N-terminal region of the Pol{alpha} protein and factors that are involved in reorganization of nucleosomes and promoting establishment of sister chromatin cohesion is required to facilitate S-phase progression.

* Corresponding author. Mailing address: Department of Pathology, Edwards Building, Room R270, Stanford University Medical Center, 300 Pasteur Dr., Stanford, CA 94305-5324. Phone: (650) 725-4907. Fax: (650) 725-4905. E-mail: tswang{at}stanford.edu.

Molecular and Cellular Biology, November 2004, p. 9568-9579, Vol. 24, No. 21
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.21.9568-9579.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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