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Molecular and Cellular Biology, November 2004, p. 10026-10035, Vol. 24, No. 22
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.22.10026-10035.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Global Chromatin Structure of 45,000 Base Pairs of Chromosome III in a- and -Cell Yeast and during Mating-Type Switching

Sevinc Ercan^* and Robert T. Simpson

Center for Gene Regulation and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania

Received 28 June 2004/ Returned for modification 19 July 2004/ Accepted 24 August 2004

Directionality of yeast mating-type switching has been attributed to differences in chromatin structure for the left arm of chromosome III. We have mapped the structure of 45 kbp of the left arm of chromosome III in a and cells in logarithmically growing cultures and in a cells during switching. Distinctive features of chromatin structure were the occurrence of DNase I-hypersensitive sites in the promoter region of nearly every gene and some replication origins and the presence of extended regions of positioned nucleosomes in 25% of the open reading frames. Other than the recombination enhancer, chromatin structures were identical in the two cell types. Changes in chromatin structure during switching were confined to the recombination enhancer. This unbiased analysis of an extended region of chromatin reveals that significant features of organized chromatin exist for the entire region, and these features are largely static with respect to mating type and mating-type switching. Our analysis also shows that primary chromatin structure does not cause the documented differences in recombinational frequency of the left arm of chromosome III in yeast a and cells.

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