This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Park, J.-E. Articles by Lee, K. S. Search for Related Content PubMed PubMed Citation Articles by Park, J.-E. Articles by Lee, K. S.

Novel Functional Dissection of the Localization-Specific Roles of Budding Yeast Polo Kinase Cdc5p

Laboratory of Metabolism,¹ Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland,⁴ Department of Biology, Dong-A University, Busan, Korea,² Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand³

Received 18 May 2004/ Accepted 30 July 2004

Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5pC) to the SPB or to the bud-neck with components specifically localizing to one of these sites and characterized these mutants in a cdc5 background. Characterization of a viable, SPB-localizing, CDC5C-CNM67 mutant revealed that it is defective in timely degradation of Swe1p, a negative regulator of Cdc28p. Loss of BFA1, a negative regulator of mitotic exit, rescued the lethality of a neck-localizing CDC5C-CDC12 or CDC5C-CDC3 mutant but yielded severe defects in cytokinesis. These data suggest that the SPB-associated Cdc5p activity is critical for both mitotic exit and cytokinesis, whereas the bud neck-localized Cdc5p is required for proper Swe1p regulation. Interestingly, a cdc5 bfa1 swe1 triple mutant is viable but grows slowly, whereas cdc5 cells bearing both CDC5C-CNM67 and CDC5C-CDC12 grow well with only a mild cell cycle delay. Thus, SPB- and the bud-neck-localized Cdc5p control most of the critical Cdc5p functions and downregulation of Bfa1p and Swe1p at the respective locations are two critical factors that require Cdc5p.

This article has been cited by other articles:

• Park, C. J., Park, J.-E., Karpova, T. S., Soung, N.-K., Yu, L.-R., Song, S., Lee, K. H., Xia, X., Kang, E., Dabanoglu, I., Oh, D.-Y., Zhang, J. Y., Kang, Y. H., Wincovitch, S., Huffaker, T. C., Veenstra, T. D., McNally, J. G., Lee, K. S. (2008). Requirement for the Budding Yeast Polo Kinase Cdc5 in Proper Microtubule Growth and Dynamics. Eukaryot Cell 7: 444-453 [Abstract] [Full Text]