This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, Y.-T. Articles by Bradley, A. Search for Related Content PubMed PubMed Citation Articles by Chen, Y.-T. Articles by Bradley, A.

Inducible Gene Trapping with Drug-Selectable Markers and Cre/loxP To Identify Developmentally Regulated Genes

Program in Developmental Biology, Baylor College of Medicine, Houston, Texas,¹ The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom²

Received 14 May 2003/ Returned for modification 24 July 2003/ Accepted 19 August 2004

Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme with drug selection markers and Cre/loxP technology which allows screening of gene trap events that responded to a signaling molecule in a 96-well format. Nine hundred twenty gene trap clones were assayed, and 258 were classified as gene traps induced by in vitro differentiation. Sixty-five of the in vitro differentiation-inducible gene traps were also responsive to retinoic acid treatment. In vivo analysis revealed that 85% of the retinoic acid-inducible gene traps trapped developmentally regulated genes, consistent with the observation that genes induced by retinoic acid treatment are likely to be developmentally regulated. Our results demonstrate that the inducible gene trapping system described here can be used to enrich in vitro for traps in genes of interest. Furthermore, we demonstrate that the cre reporter is extremely sensitive and can be used to explore chromosomal regions that are not detectable with neo as a selection cassette.

This article has been cited by other articles:

• Cho, Y.-S., Kim, E.-J., Park, U.-H., Sin, H.-S., Um, S.-J. (2006). Additional Sex Comb-like 1 (ASXL1), in Cooperation with SRC-1, Acts as a Ligand-dependent Coactivator for Retinoic Acid Receptor. J. Biol. Chem. 281: 17588-17598 [Abstract] [Full Text]  
• Topaloglu, O., Hurley, P. J., Yildirim, O., Civin, C. I., Bunz, F. (2005). Improved methods for the generation of human gene knockout and knockin cell lines. Nucleic Acids Res 33: e158-e158 [Abstract] [Full Text]