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Molecular and Cellular Biology, December 2004, p. 10894-10904, Vol. 24, No. 24
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.24.10894-10904.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nonphosphorylated Human La Antigen Interacts with Nucleolin at Nucleolar Sites Involved in rRNA Biogenesis

Robert V. Intine,1,{dagger} Miroslav Dundr,2,{dagger} Alex Vassilev,1 Elena Schwartz,1 Yingmin Zhao,3 Yingxin Zhao,3 Melvin L. DePamphilis,1 and Richard J. Maraia1*

Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development,1 National Cancer Institute, Bethesda, Maryland,2 Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas3

Received 30 July 2004/ Returned for modification 20 August 2004/ Accepted 17 September 2004

La is a RNA-binding protein implicated in multiple pathways related to the production of tRNAs, ribosomal proteins, and other components of the translational machinery (D. J. Kenan and J. D. Keene, Nat. Struct. Mol. Biol. 11:303-305, 2004). While most La is phosphorylated and resides in the nucleoplasm, a fraction is in the nucleolus, the site of ribosome production, although the determinants of this localization are incompletely known. In addition to its conserved N-terminal domain, human La harbors a C-terminal domain that contains an atypical RNA recognition motif and a short basic motif (SBM) adjacent to phosphoserine-366. We report that nonphosphorylated La (npLa) is concentrated in nucleolar sites that correspond to the dense fibrillar component that harbors nascent pol I transcripts as well as fibrillarin and nucleolin, which function in early phases of rRNA maturation. Affinity purification and native immunoprecipitation of La and fluorescence resonance energy transfer in the nucleolus reveal close association with nucleolin. Moreover, La lacking the SBM does not localize to nucleoli. Lastly, La exhibits SBM-dependent, phosphorylation-sensitive interaction with nucleolin in a yeast two-hybrid assay. The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis.

* Corresponding author. Mailing address: Laboratory of Molecular Growth Regulation, National Institute of Child Health & Human Development, 6 Center Dr., Rm. 416, Bethesda, MD 20892-2753. Phone: (301) 402-3567. Fax: (301) 480-6863. E-mail: maraiar{at}mail.nih.gov.

{dagger} R.V.I. and M.D. contributed equally to the present study.

Molecular and Cellular Biology, December 2004, p. 10894-10904, Vol. 24, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.24.10894-10904.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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