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Molecular and Cellular Biology, December 2004, p. 10965-10974, Vol. 24, No. 24
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.24.10965-10974.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evidence for Histone Eviction in trans upon Induction of the Yeast PHO5 Promoter{dagger}

Philipp Korber,* Tim Luckenbach, Dorothea Blaschke, and Wolfram Hörz

Adolf-Butenandt-Institut, Universität München, Munich, Germany

Received 1 July 2004/ Returned for modification 28 July 2004/ Accepted 20 September 2004

The yeast PHO5 promoter is a model system for the role of chromatin in eukaryotic gene regulation. Four positioned nucleosomes in the repressed state give way to an extended DNase I hypersensitive site upon induction. Recently this hypersensitive site was shown to be devoid of histone DNA contacts. This raises the mechanistic question of how histones are removed from the promoter. A displacement in trans or movement in cis, the latter according to the well established nucleosome sliding mechanism, are the major alternatives. In this study, we embedded the PHO5 promoter into the context of a small plasmid which severely restricts the space for nucleosome sliding along the DNA in cis. Such a construct would either preclude the chromatin transition upon induction altogether, were it to occur in cis, or gross changes in chromatin around the plasmid would be the consequence. We observed neither. Instead, promoter opening on the plasmid was indistinguishable from opening at the native chromosomal locus. This makes a sliding mechanism for the chromatin transition at the PHO5 promoter highly unlikely and points to histone eviction in trans.

* Corresponding author. Mailing address: Adolf-Butenandt-Institut, Universität München, Schillerstr. 44, 80336 Munich, Germany. Phone: 49-89-218075779. Fax: 49-89-218075440. E-mail: pkorber{at}lmu.de.

{dagger} This paper is dedicated to Mark N. Hoover Thames and Viticia A. Thames on the occasion of their wedding.

Molecular and Cellular Biology, December 2004, p. 10965-10974, Vol. 24, No. 24
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.24.10965-10974.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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