This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Forget, D.
Right arrow Articles by Coulombe, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Forget, D.
Right arrow Articles by Coulombe, B.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2004, p. 1122-1131, Vol. 24, No. 3
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.3.1122-1131.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Photo-Cross-Linking of a Purified Preinitiation Complex Reveals Central Roles for the RNA Polymerase II Mobile Clamp and TFIIE in Initiation Mechanisms

Diane Forget, Marie-France Langelier, Cynthia Thérien, Vincent Trinh, and Benoit Coulombe*

Laboratory of Gene Transcription, Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada H2W 1R7

Received 24 September 2003/ Accepted 3 November 2003

The topological organization of a TATA binding protein-TFIIB-TFIIF-RNA polymerase II (RNAP II)-TFIIE-promoter complex was analyzed using site-specific protein-DNA photo-cross-linking of gel-purified complexes. The cross-linking results for the subunits of RNAP II were used to determine the path of promoter DNA against the structure of the enzyme. The results indicate that promoter DNA wraps around the mobile clamp of RNAP II. Cross-linking of TFIIF and TFIIE both upstream of the TATA element and downstream of the transcription start site suggests that both factors associate with the RNAP II mobile clamp. TFIIE{alpha} closely approaches promoter DNA at nucleotide -10, a position immediately upstream of the transcription bubble in the open complex. Increased stimulation of transcription initiation by TFIIE{alpha} is obtained when the DNA template is artificially premelted in the -11/-1 region, suggesting that TFIIE{alpha} facilitates open complex formation, possibly through its interaction with the upstream end of the partially opened transcription bubble. These results support the central roles of the mobile clamp of RNAP II and TFIIE in transcription initiation.

* Corresponding author. Mailing address: Laboratory of Gene Transcription, Institut de Recherches Cliniques de Montréal, 110 Ave. des Pins Ouest, Montréal, Québec, Canada H2W 1R7. Phone: (514) 987-5662. Fax: (514) 987-5663. E-mail: coulomb{at}ircm.qc.ca.

Molecular and Cellular Biology, February 2004, p. 1122-1131, Vol. 24, No. 3
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.3.1122-1131.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Micorescu, M., Grunberg, S., Franke, A., Cramer, P., Thomm, M., Bartlett, M. (2008). Archaeal Transcription: Function of an Alternative Transcription Factor B from Pyrococcus furiosus. J. Bacteriol. 190: 157-167 [Abstract] [Full Text]  
  • Grunberg, S., Bartlett, M. S., Naji, S., Thomm, M. (2007). Transcription Factor E Is a Part of Transcription Elongation Complexes. J. Biol. Chem. 282: 35482-35490 [Abstract] [Full Text]  
  • Davis, C. A., Bingman, C. A., Landick, R., Record, M. T. Jr., Saecker, R. M. (2007). Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase. Proc. Natl. Acad. Sci. USA 104: 7833-7838 [Abstract] [Full Text]  
  • Naji, S., Grunberg, S., Thomm, M. (2007). The RPB7 Orthologue E' Is Required for Transcriptional Activity of a Reconstituted Archaeal Core Enzyme at Low Temperatures and Stimulates Open Complex Formation. J. Biol. Chem. 282: 11047-11057 [Abstract] [Full Text]  
  • Trinh, V., Langelier, M.-F., Archambault, J., Coulombe, B. (2006). Structural Perspective on Mutations Affecting the Function of Multisubunit RNA Polymerases. Microbiol. Mol. Biol. Rev. 70: 12-36 [Abstract] [Full Text]  
  • Vojnic, E., Simon, B., Strahl, B. D., Sattler, M., Cramer, P. (2006). Structure and Carboxyl-terminal Domain (CTD) Binding of the Set2 SRI Domain That Couples Histone H3 Lys36 Methylation to Transcription. J. Biol. Chem. 281: 13-15 [Abstract] [Full Text]  
  • Tang, G.-Q., Bandwar, R. P., Patel, S. S. (2005). Extended Upstream A-T Sequence Increases T7 Promoter Strength. J. Biol. Chem. 280: 40707-40713 [Abstract] [Full Text]  
  • Werner, F., Weinzierl, R. O. J. (2005). Direct Modulation of RNA Polymerase Core Functions by Basal Transcription Factors. Mol. Cell. Biol. 25: 8344-8355 [Abstract] [Full Text]  
  • Le, T. T. T., Zhang, S., Hayashi, N., Yasukawa, M., Delgermaa, L., Murakami, S. (2005). Mutational Analysis of Human RNA Polymerase II Subunit 5 (RPB5): The Residues Critical for Interactions with TFIIF Subunit RAP30 and Hepatitis B Virus X Protein. J Biochem 138: 215-224 [Abstract] [Full Text]  
  • Hayashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., Kimura, M., Yokoi, M., Ishihama, A., Hanaoka, F., Ohkuma, Y. (2005). Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation. GENES CELLS 10: 207-224 [Abstract] [Full Text]  
  • Ouhammouch, M., Werner, F., Weinzierl, R. O. J., Geiduschek, E. P. (2004). A Fully Recombinant System for Activator-dependent Archaeal Transcription. J. Biol. Chem. 279: 51719-51721 [Abstract] [Full Text]  
  • Coulombe, B., Jeronimo, C., Langelier, M.-F., Cojocaru, M., Bergeron, D. (2004). Interaction Networks of the Molecular Machines That Decode, Replicate, and Maintain the Integrity of the Human Genome. Mol. Cell. Proteomics 3: 851-856 [Abstract] [Full Text]  
  • Jeronimo, C., Langelier, M.-F., Zeghouf, M., Cojocaru, M., Bergeron, D., Baali, D., Forget, D., Mnaimneh, S., Davierwala, A. P., Pootoolal, J., Chandy, M., Canadien, V., Beattie, B. K., Richards, D. P., Workman, J. L., Hughes, T. R., Greenblatt, J., Coulombe, B. (2004). RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits. Mol. Cell. Biol. 24: 7043-7058 [Abstract] [Full Text]  
  • Tapias, A., Auriol, J., Forget, D., Enzlin, J. H., Scharer, O. D., Coin, F., Coulombe, B., Egly, J.-M. (2004). Ordered Conformational Changes in Damaged DNA Induced by Nucleotide Excision Repair Factors. J. Biol. Chem. 279: 19074-19083 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»