This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lahlil, R.
Right arrow Articles by Hoang, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lahlil, R.
Right arrow Articles by Hoang, T.

Next Article 

Molecular and Cellular Biology, February 2004, p. 1439-1452, Vol. 24, No. 4
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.4.1439-1452.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SCL Assembles a Multifactorial Complex That Determines Glycophorin A Expression

Rachid Lahlil, Eric Lécuyer, Sabine Herblot, and Trang Hoang*

Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, and Departments of Pharmacology, Biochemistry, and Molecular Biology and Institute of Immunovirology and Cancer, University of Montréal, Montréal, Québec H3C 3J7, Canada

Received 7 July 2003/ Returned for modification 22 August 2003/ Accepted 7 November 2003

SCL/TAL1 is a hematopoietic-specific transcription factor of the basic helix-loop-helix (bHLH) family that is essential for erythropoiesis. Here we identify the erythroid cell-specific glycophorin A gene (GPA) as a target of SCL in primary hematopoietic cells and show that SCL occupies the GPA locus in vivo. GPA promoter activation is dependent on the assembly of a multifactorial complex containing SCL as well as ubiquitous (E47, Sp1, and Ldb1) and tissue-specific (LMO2 and GATA-1) transcription factors. In addition, our observations suggest functional specialization within this complex, as SCL provides its HLH protein interaction motif, GATA-1 exerts a DNA-tethering function through its binding to a critical GATA element in the GPA promoter, and E47 requires its N-terminal moiety (most likely entailing a transactivation function). Finally, endogenous GPA expression is disrupted in hematopoietic cells through the dominant-inhibitory effect of a truncated form of E47 (E47-bHLH) on E-protein activity or of FOG (Friend of GATA) on GATA activity or when LMO2 or Ldb-1 protein levels are decreased. Together, these observations reveal the functional complementarities of transcription factors within the SCL complex and the essential role of SCL as a nucleation factor within a higher-order complex required to activate gene GPA expression.

* Corresponding author. Mailing address: Institut de Recherche en Immunovirologie et Cancérologie, Université de Montréal, P.O. Box 6128, Centre-ville Branch, Montréal, Québec H3C 2J7, Canada. Phone: (514) 343-6870. Fax: (514) 343-6945. E-mail: trang.hoang{at}umontreal.ca.

Molecular and Cellular Biology, February 2004, p. 1439-1452, Vol. 24, No. 4
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.4.1439-1452.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Terme, J.-M., Lhermitte, L., Asnafi, V., Jalinot, P. (2009). TGF-{beta} induces degradation of TAL1/SCL by the ubiquitin-proteasome pathway through AKT-mediated phosphorylation. Blood 113: 6695-6698 [Abstract] [Full Text]  
  • Appert, A., Nam, C.-H., Lobato, N., Priego, E., Miguel, R. N., Blundell, T., Drynan, L., Sewell, H., Tanaka, T., Rabbitts, T. (2009). Targeting LMO2 with a Peptide Aptamer Establishes a Necessary Function in Overt T-Cell Neoplasia. Cancer Res. 69: 4784-4790 [Abstract] [Full Text]  
  • Gekas, C., Rhodes, K. E., Gereige, L. M., Helgadottir, H., Ferrari, R., Kurdistani, S. K., Montecino-Rodriguez, E., Bassel-Duby, R., Olson, E., Krivtsov, A. V., Armstrong, S., Orkin, S. H., Pellegrini, M., Mikkola, H. K. A. (2009). Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis. Blood 113: 3461-3471 [Abstract] [Full Text]  
  • Felli, N., Pedini, F., Romania, P., Biffoni, M., Morsilli, O., Castelli, G., Santoro, S., Chicarella, S., Sorrentino, A., Peschle, C., Marziali, G. (2009). MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis. haematol 94: 479-486 [Abstract] [Full Text]  
  • Tripic, T., Deng, W., Cheng, Y., Zhang, Y., Vakoc, C. R., Gregory, G. D., Hardison, R. C., Blobel, G. A. (2009). SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood 113: 2191-2201 [Abstract] [Full Text]  
  • Wozniak, R. J., Keles, S., Lugus, J. J., Young, K. H., Boyer, M. E., Tran, T. M., Choi, K., Bresnick, E. H. (2008). Molecular Hallmarks of Endogenous Chromatin Complexes Containing Master Regulators of Hematopoiesis. Mol. Cell. Biol. 28: 6681-6694 [Abstract] [Full Text]  
  • Hamlett, I., Draper, J., Strouboulis, J., Iborra, F., Porcher, C., Vyas, P. (2008). Characterization of megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to regulate terminal megakaryocyte maturation. Blood 112: 2738-2749 [Abstract] [Full Text]  
  • Grosso, A. R., Gomes, A. Q., Barbosa-Morais, N. L., Caldeira, S., Thorne, N. P., Grech, G., von Lindern, M., Carmo-Fonseca, M. (2008). Tissue-specific splicing factor gene expression signatures. Nucleic Acids Res 36: 4823-4832 [Abstract] [Full Text]  
  • Terme, J.-M., Wencker, M., Favre-Bonvin, A., Bex, F., Gazzolo, L., Duc Dodon, M., Jalinot, P. (2008). Cross Talk between Expression of the Human T-Cell Leukemia Virus Type 1 Tax Transactivator and the Oncogenic bHLH Transcription Factor TAL1. J. Virol. 82: 7913-7922 [Abstract] [Full Text]  
  • Zenvirt, S., Nevo-Caspi, Y., Rencus-Lazar, S., Segal, D. (2008). Drosophila LIM-Only Is a Positive Regulator of Transcription During Thoracic Bristle Development. Genetics 179: 1989-1999 [Abstract] [Full Text]  
  • Landry, J.-R., Kinston, S., Knezevic, K., de Bruijn, M. F.T.R., Wilson, N., Nottingham, W. T., Peitz, M., Edenhofer, F., Pimanda, J. E., Ottersbach, K., Gottgens, B. (2008). Runx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver. Blood 111: 3005-3014 [Abstract] [Full Text]  
  • De Gobbi, M., Anguita, E., Hughes, J., Sloane-Stanley, J. A., Sharpe, J. A., Koch, C. M., Dunham, I., Gibbons, R. J., Wood, W. G., Higgs, D. R. (2007). Tissue-specific histone modification and transcription factor binding in {alpha} globin gene expression. Blood 110: 4503-4510 [Abstract] [Full Text]  
  • Lecuyer, E., Lariviere, S., Sincennes, M.-C., Haman, A., Lahlil, R., Todorova, M., Tremblay, M., Wilkes, B. C., Hoang, T. (2007). Protein Stability and Transcription Factor Complex Assembly Determined by the SCL-LMO2 Interaction. J. Biol. Chem. 282: 33649-33658 [Abstract] [Full Text]  
  • Ogilvy, S., Ferreira, R., Piltz, S. G., Bowen, J. M., Gottgens, B., Green, A. R. (2007). The SCL +40 Enhancer Targets the Midbrain Together with Primitive and Definitive Hematopoiesis and Is Regulated by SCL and GATA Proteins. Mol. Cell. Biol. 27: 7206-7219 [Abstract] [Full Text]  
  • Wozniak, R. J., Boyer, M. E., Grass, J. A., Lee, Y., Bresnick, E. H. (2007). Context-dependent GATA Factor Function: COMBINATORIAL REQUIREMENTS FOR TRANSCRIPTIONAL CONTROL IN HEMATOPOIETIC AND ENDOTHELIAL CELLS. J. Biol. Chem. 282: 14665-14674 [Abstract] [Full Text]  
  • Deleuze, V., Chalhoub, E., El-Hajj, R., Dohet, C., Le Clech, M., Couraud, P.-O., Huber, P., Mathieu, D. (2007). TAL-1/SCL and Its Partners E47 and LMO2 Up-Regulate VE-Cadherin Expression in Endothelial Cells. Mol. Cell. Biol. 27: 2687-2697 [Abstract] [Full Text]  
  • Patterson, L. J., Gering, M., Eckfeldt, C. E., Green, A. R., Verfaillie, C. M., Ekker, S. C., Patient, R. (2007). The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish. Blood 109: 2389-2398 [Abstract] [Full Text]  
  • Donaldson, I. J., Gottgens, B. (2007). CoMoDis: composite motif discovery in mammalian genomes. Nucleic Acids Res 35: e1-e1 [Abstract] [Full Text]  
  • Meier, N., Krpic, S., Rodriguez, P., Strouboulis, J., Monti, M., Krijgsveld, J., Gering, M., Patient, R., Hostert, A., Grosveld, F. (2006). Novel binding partners of Ldb1 are required for haematopoietic development. Development 133: 4913-4923 [Abstract] [Full Text]  
  • de la Grange, P. B., Armstrong, F., Duval, V., Rouyez, M.-C., Goardon, N., Romeo, P.-H., Pflumio, F. (2006). Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells. Blood 108: 2998-3004 [Abstract] [Full Text]  
  • Li, X., Xiong, J.-W., Shelley, C. S., Park, H., Arnaout, M. A. (2006). The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells. Development 133: 3641-3650 [Abstract] [Full Text]  
  • Palomero, T., Odom, D. T., O'Neil, J., Ferrando, A. A., Margolin, A., Neuberg, D. S., Winter, S. S., Larson, R. S., Li, W., Liu, X. S., Young, R. A., Look, A. T. (2006). Transcriptional regulatory networks downstream of TAL1/SCL in T-cell acute lymphoblastic leukemia. Blood 108: 986-992 [Abstract] [Full Text]  
  • Suh, H. C., Gooya, J., Renn, K., Friedman, A. D., Johnson, P. F., Keller, J. R. (2006). C/EBP{alpha} determines hematopoietic cell fate in multipotential progenitor cells by inhibiting erythroid differentiation and inducing myeloid differentiation. Blood 107: 4308-4316 [Abstract] [Full Text]  
  • Landry, J.-R., Kinston, S., Knezevic, K., Donaldson, I. J., Green, A. R., Gottgens, B. (2005). Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells. Blood 106: 2680-2687 [Abstract] [Full Text]  
  • Hall, M. A., Slater, N. J., Begley, C. G., Salmon, J. M., Van Stekelenburg, L. J., McCormack, M. P., Jane, S. M., Curtis, D. J. (2005). Functional but Abnormal Adult Erythropoiesis in the Absence of the Stem Cell Leukemia Gene. Mol. Cell. Biol. 25: 6355-6362 [Abstract] [Full Text]  
  • Zhang, Y., Payne, K. J., Zhu, Y., Price, M. A., Parrish, Y. K., Zielinska, E., Barsky, L. W., Crooks, G. M. (2005). SCL Expression at Critical Points in Human Hematopoietic Lineage Commitment. Stem Cells 23: 852-860 [Abstract] [Full Text]  
  • Wen, J., Huang, S., Pack, S. D., Yu, X., Brandt, S. J., Noguchi, C. T. (2005). Tal1/SCL Binding to Pericentromeric DNA Represses Transcription. J. Biol. Chem. 280: 12956-12966 [Abstract] [Full Text]  
  • Valverde-Garduno, V., Guyot, B., Anguita, E., Hamlett, I., Porcher, C., Vyas, P. (2004). Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification. Blood 104: 3106-3116 [Abstract] [Full Text]  
  • Ravet, E., Reynaud, D., Titeux, M., Izac, B., Fichelson, S., Romeo, P.-H., Dubart-Kupperschmitt, A., Pflumio, F. (2004). Characterization of DNA-binding-dependent and -independent functions of SCL/TAL1 during human erythropoiesis. Blood 103: 3326-3335 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»