This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Damianov, A. Articles by Bindereif, A. Search for Related Content PubMed PubMed Citation Articles by Damianov, A. Articles by Bindereif, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2004, p. 1700-1708, Vol. 24, No. 4
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.4.1700-1708.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Recycling of the U12-Type Spliceosome Requires p110, a Component of the U6atac snRNP

Institut für Biochemie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany

Received 24 June 2003/ Returned for modification 11 August 2003/ Accepted 17 November 2003

U12-dependent introns are spliced by the so-called minor spliceosome, requiring the U11, U12, and U4atac/U6atac snRNPs in addition to the U5 snRNP. We have recently identified U6-p110 (SART3) as a novel human recycling factor that is related to the yeast splicing factor Prp24. U6-p110 transiently associates with the U6 and U4/U6 snRNPs during the spliceosome cycle, regenerating functional U4/U6 snRNPs from singular U4 and U6 snRNPs. Here we investigated the involvement of U6-p110 in recycling of the U4atac/U6atac snRNP. In contrast to the major U6 and U4/U6 snRNPs, p110 is primarily associated with the U6atac snRNP but is almost undetectable in the U4atac/U6atac snRNP. Since p110 does not occur in U5 snRNA-containing complexes, it appears to be transiently associated with U6atac during the cycle of the minor spliceosome. The p110 binding site was mapped to U6 nucleotides 38 to 57 and U6atac nucleotides 10 to 30, which are highly conserved between these two functionally related snRNAs. With a U12-dependent in vitro splicing system, we demonstrate that p110 is required for recycling of the U4atac/U6atac snRNP.

This article has been cited by other articles:

• Licht, K., Medenbach, J., Luhrmann, R., Kambach, C., Bindereif, A. (2008). 3'-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins. RNA 14: 1532-1538 [Abstract] [Full Text]  
• Trede, N. S., Medenbach, J., Damianov, A., Hung, L.-H., Weber, G. J., Paw, B. H., Zhou, Y., Hersey, C., Zapata, A., Keefe, M., Barut, B. A., Stuart, A. B., Katz, T., Amemiya, C. T., Zon, L. I., Bindereif, A. (2007). Network of coregulated spliceosome components revealed by zebrafish mutant in recycling factor p110. Proc. Natl. Acad. Sci. USA 104: 6608-6613 [Abstract] [Full Text]  
• Trippe, R., Guschina, E., Hossbach, M., Urlaub, H., Luhrmann, R., Benecke, B.-J. (2006). Identification, cloning, and functional analysis of the human U6 snRNA-specific terminal uridylyl transferase. RNA 12: 1494-1504 [Abstract] [Full Text]  
• KWAN, S. S., BROW, D. A. (2005). The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding. RNA 11: 808-820 [Abstract] [Full Text]  
• Stanek, D., Neugebauer, K. M. (2004). Detection of snRNP assembly intermediates in Cajal bodies by fluorescence resonance energy transfer. J. Cell Biol. 166: 1015-1025 [Abstract] [Full Text]  
• Medenbach, J., Schreiner, S., Liu, S., Luhrmann, R., Bindereif, A. (2004). Human U4/U6 snRNP Recycling Factor p110: Mutational Analysis Reveals the Function of the Tetratricopeptide Repeat Domain in Recycling. Mol. Cell. Biol. 24: 7392-7401 [Abstract] [Full Text]