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Molecular and Cellular Biology, March 2004, p. 2091-2102, Vol. 24, No. 5
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.5.2091-2102.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cell Cycle Localization, Dimerization, and Binding Domain Architecture of the Telomere Protein cPot1

Chao Wei and Carolyn M. Price*

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524

Received 10 October 2003/ Returned for modification 21 November 2003/ Accepted 12 December 2003

Pot1 is a single-stranded-DNA-binding protein that recognizes telomeric G-strand DNA. It is essential for telomere capping in Saccharomyces pombe and regulates telomere length in humans. Human Pot1 also interacts with proteins that bind the duplex region of the telomeric tract. Thus, like Cdc13 from S. cerevisiae, Pot 1 may have multiple roles at the telomere. We show here that endogenous chicken Pot1 (cPot1) is present at telomeres during periods of the cell cycle when t loops are thought to be present. Since cPot1 can bind internal loops and directly adjacent DNA-binding sites, it is likely to fully coat and protect both G-strand overhangs and the displaced G strand of a t loop. The minimum binding site of cPot1 is double that of the S. pombe DNA-binding domain. Although cPot can self associate, dimerization is not required for DNA binding and hence does not explain the binding-site duplication. Instead, the DNA-binding domain appears to be extended to contain a second binding motif in addition to the conserved oligonucleotide-oligosaccharide (OB) fold present in other G-strand-binding proteins. This second motif could be another OB fold. Although dimerization is inefficient in vitro, it may be regulated in vivo and could promote association with other telomere proteins and/or telomere compaction.

* Corresponding author. Mailing address: Dept. of Molecular Genetics, Biochemistry, and Microbiology, College of Medicine, University of Cincinnati, ML0524 231 Albert Sabin Way, Cincinnati, OH 45267. Phone (513) 558-0450. Fax: (513) 558-8474. E-mail: Carolyn.Price{at}uc.edu.

Molecular and Cellular Biology, March 2004, p. 2091-2102, Vol. 24, No. 5
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.5.2091-2102.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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