This Article Full Text Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, R. Articles by Leptin, M. Search for Related Content PubMed PubMed Citation Articles by Wilson, R. Articles by Leptin, M.

A Functional Domain of Dof That Is Required for Fibroblast Growth Factor Signaling

Robert Wilson, Alysia Battersby,^1, Agnes Csiszar, Elisabeth Vogelsang, and Maria Leptin^*

Institut für Genetik, Universität zu Köln, Weyertal 121, D-50931 Cologne, Germany

Received 3 October 2003/ Accepted 11 December 2003

Signal transduction by fibroblast growth factor (FGF) receptors in Drosophila depends upon the intracellular protein Dof, which has been proposed to act downstream of the receptors and upstream of Ras. Dof is the product of a fast-evolving gene whose vertebrate homologs, BCAP and BANK, are involved in signaling downstream of the B-cell receptor. Mapping functional domains within Dof revealed that neither of its potential interaction motifs, the ankyrin repeats and the coiled coil, is essential for the function of Dof. However, we have identified a region within the N terminus of the protein with similarity to BCAP and BANK, which we refer to as the Dof, BCAP, and BANK (DBB) motif, that it is required for FGF-dependent signal transduction and is necessary for efficient interaction of Dof with the FGF receptor Heartless. In addition, we demonstrate that Dof is phosphorylated in the presence of an activated FGF receptor and that tyrosine residues could contribute to the function of the molecule.

Present address: School of Biological Sciences, University of Wales—Bangor, Bangor LL57 2UW, United Kingdom.

Supplemental material for this article may be found at http://mcb.asm.org/.

This article has been cited by other articles:

• Affolter, M., Caussinus, E. (2008). Tracheal branching morphogenesis in Drosophila: new insights into cell behaviour and organ architecture. Development 135: 2055-2064 [Abstract] [Full Text]  
• Toledano-Katchalski, H., Nir, R., Volohonsky, G., Volk, T. (2007). Post-transcriptional repression of the Drosophila midkine and pleiotrophin homolog miple by HOW is essential for correct mesoderm spreading. Development 134: 3473-3481 [Abstract] [Full Text]  
• Zhu, M. Y., Wilson, R., Leptin, M. (2005). A Screen for Genes That Influence Fibroblast Growth Factor Signal Transduction in Drosophila. Genetics 170: 767-777 [Abstract] [Full Text]  
• Wilson, R., Vogelsang, E., Leptin, M. (2005). FGF signalling and the mechanism of mesoderm spreading in Drosophila embryos. Development 132: 491-501 [Abstract] [Full Text]  
• Cabernard, C., Neumann, M., Affolter, M. (2004). Cellular and molecular mechanisms involved in branching morphogenesis of the Drosophila tracheal system. J. Appl. Physiol. 97: 2347-2353 [Abstract] [Full Text]