This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Geymonat, M.
Right arrow Articles by Sedgwick, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geymonat, M.
Right arrow Articles by Sedgwick, S. G.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2004, p. 2277-2285, Vol. 24, No. 6
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.6.2277-2285.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Clb6/Cdc28 and Cdc14 Regulate Phosphorylation Status and Cellular Localization of Swi6

Marco Geymonat,1,{dagger} Ad Spanos,1,{dagger} Glenn P. Wells,1,{dagger},{ddagger} Stephen J. Smerdon,2 and Steven G. Sedgwick1*

Divisions of Yeast Genetics,1 Protein Structure, National Institute for Medical Research, London NW7 1AA, United Kingdom2

Received 9 October 2003/ Returned for modification 17 November 2003/ Accepted 29 December 2003

Nuclear export of the transcription factor Swi6 during the budding yeast Saccharomyces cerevisiae cell cycle is known to require phosphorylation of the Swi6 serine 160 residue. We show that Clb6/Cdc28 kinase is required for this nuclear export. Furthermore, Cdc28 combined with the S-phase cyclin Clb6 specifically phosphorylates serine 160 of Swi6 in vitro. Nuclear import of Swi6 occurs concomitantly with dephosphorylation of serine 160 in late M phase. We show that Cdc14 phosphatase, the principal effector of the mitotic exit network, can trigger nuclear import of Swi6 in vivo and that Cdc14 dephosphorylates Swi6 at serine 160 in vitro. Taken together, these observations show how Swi6 dephosphorylation and phosphorylation are integrated into changes of Cdc28 activity governing entry and exit from the G1 phase of the cell cycle.


* Corresponding author. Mailing address: National Institute for Medical Research, London NW7 1AA, United Kingdom. Phone: 0208 816 2246. Fax: 0208 816 2523. E-mail: ssedgwi{at}nimr.mrc.ac.uk.

{dagger} M.G., A.S., and G.P.W. contributed equally to this work.

{ddagger} Present address: The Wellcome Trust, London NW1 2BE, United Kingdom


Molecular and Cellular Biology, March 2004, p. 2277-2285, Vol. 24, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.6.2277-2285.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Dulev, S., de Renty, C., Mehta, R., Minkov, I., Schwob, E., Strunnikov, A. (2009). Essential global role of CDC14 in DNA synthesis revealed by chromosome underreplication unrecognized by checkpoints in cdc14 mutants. Proc. Natl. Acad. Sci. USA 106: 14466-14471 [Abstract] [Full Text]  
  • Mohl, D. A., Huddleston, M. J., Collingwood, T. S., Annan, R. S., Deshaies, R. J. (2009). Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. JCB 184: 527-539 [Abstract] [Full Text]  
  • Kim, K.-Y., Truman, A. W., Levin, D. E. (2008). Yeast Mpk1 Mitogen-Activated Protein Kinase Activates Transcription through Swi4/Swi6 by a Noncatalytic Mechanism That Requires Upstream Signal. Mol. Cell. Biol. 28: 2579-2589 [Abstract] [Full Text]  
  • Braun, K. A., Breeden, L. L. (2007). Nascent Transcription of MCM2-7 Is Important for Nuclear Localization of the Minichromosome Maintenance Complex in G1. Mol. Biol. Cell 18: 1447-1456 [Abstract] [Full Text]  
  • Bloom, J., Cross, F. R. (2007). Novel Role for Cdc14 Sequestration: Cdc14 Dephosphorylates Factors That Promote DNA Replication. Mol. Cell. Biol. 27: 842-853 [Abstract] [Full Text]  
  • Jackson, L. P., Reed, S. I., Haase, S. B. (2006). Distinct mechanisms control the stability of the related s-phase cyclins clb5 and clb6.. Mol. Cell. Biol. 26: 2456-2466 [Abstract] [Full Text]  
  • Liku, M. E., Nguyen, V. Q., Rosales, A. W., Irie, K., Li, J. J. (2005). CDK Phosphorylation of a Novel NLS-NES Module Distributed between Two Subunits of the Mcm2-7 Complex Prevents Chromosomal Rereplication. Mol. Biol. Cell 16: 5026-5039 [Abstract] [Full Text]