This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Major, M. L.
Right arrow Articles by Costa, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Major, M. L.
Right arrow Articles by Costa, R. H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2004, p. 2649-2661, Vol. 24, No. 7
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.7.2649-2661.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Forkhead Box M1B Transcriptional Activity Requires Binding of Cdk-Cyclin Complexes for Phosphorylation-Dependent Recruitment of p300/CBP Coactivators

Michael L. Major, Rita Lepe, and Robert H. Costa*

Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60607

Received 15 August 2003/ Returned for modification 18 November 2003/ Accepted 9 January 2004

Previous liver regeneration studies demonstrated that the mouse forkhead box M1B (FoxM1B) transcription factor regulates hepatocyte proliferation through expression of cell cycle genes that stimulate cyclin-dependent kinase 2 (Cdk2) and Cdk1 activity. In this study, we demonstrated that disruption of the FoxM1B Cdk1/2 phosphorylation site at Thr residue 596 significantly reduced both FoxM1B transcriptional activity and Cdk phosphorylation of the FoxM1B T596A mutant protein in vivo. Retention of this FoxM1B 596 Cdk phosphorylation site was found to be essential for recruiting the histone acetyltransferase CREB binding protein (CBP) to the FoxM1B transcriptional activation domain. Consistent with these findings, dominant negative Cdk1 protein significantly reduced FoxM1B transcriptional activity and inhibited FoxM1B recruitment of the CBP coactivator protein. Likewise, Cdc25B-mediated stimulation of Cdk activity together with elevated levels of the CBP coactivator protein provided a 6.2-fold synergistic increase in FoxM1B transcriptional activity. Furthermore, mutation of the FoxM1B Leu 641 residue within an LXL motif (residues 639 to 641) inhibited recruitment of Cdk-cyclin complexes and caused significant reduction in both FoxM1B transcriptional activity and in vivo Cdk phosphorylation of the FoxM1B Thr 596 residue. We demonstrated that FoxM1B transcriptional activity requires binding of either S-phase or M-phase Cdk-cyclin complexes to mediate efficient Cdk phosphorylation of the FoxM1B Thr 596 residue, which is essential for recruitment of p300/CBP coactivator proteins.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Genetics (M/C 669), University of Illinois at Chicago, College of Medicine, 900 S. Ashland Ave., Rm. 2220 MBRB, Chicago, IL 60607-7170. Phone: (312) 996-0474. Fax: (312) 355-4010. E-mail: RobCosta{at}uic.edu.

Molecular and Cellular Biology, April 2004, p. 2649-2661, Vol. 24, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.7.2649-2661.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, F.-J., Barchowsky, A., Opresko, P. L. (2009). The Werner Syndrome Protein Functions in Repair of Cr(VI)-Induced Replication-Associated DNA Damage. Toxicol Sci 110: 307-318 [Abstract] [Full Text]  
  • Stoyanova, T., Roy, N., Kopanja, D., Bagchi, S., Raychaudhuri, P. (2009). DDB2 decides cell fate following DNA damage. Proc. Natl. Acad. Sci. USA 106: 10690-10695 [Abstract] [Full Text]  
  • Calvisi, D F, Pinna, F, Ladu, S, Pellegrino, R, Simile, M M, Frau, M, De Miglio, M R, Tomasi, M L, Sanna, V, Muroni, M R, Feo, F, Pascale, R M (2009). Forkhead box M1B is a determinant of rat susceptibility to hepatocarcinogenesis and sustains ERK activity in human HCC. Gut 58: 679-687 [Abstract] [Full Text]  
  • Lindqvist, A., Rodriguez-Bravo, V., Medema, R. H. (2009). The decision to enter mitosis: feedback and redundancy in the mitotic entry network. JCB 185: 193-202 [Abstract] [Full Text]  
  • Li, Q., Zhang, N., Jia, Z., Le, X., Dai, B., Wei, D., Huang, S., Tan, D., Xie, K. (2009). Critical Role and Regulation of Transcription Factor FoxM1 in Human Gastric Cancer Angiogenesis and Progression. Cancer Res. 69: 3501-3509 [Abstract] [Full Text]  
  • McGovern, U. B., Francis, R. E., Peck, B., Guest, S. K., Wang, J., Myatt, S. S., Krol, J., Kwok, J. M-M., Polychronis, A., Coombes, R. C., Lam, E. W-F. (2009). Gefitinib (Iressa) represses FOXM1 expression via FOXO3a in breast cancer. Molecular Cancer Therapeutics 8: 582-591 [Abstract] [Full Text]  
  • Park, H. J., Costa, R. H., Lau, L. F., Tyner, A. L., Raychaudhuri, P. (2008). Anaphase-Promoting Complex/Cyclosome-Cdh1-Mediated Proteolysis of the Forkhead Box M1 Transcription Factor Is Critical for Regulated Entry into S Phase. Mol. Cell. Biol. 28: 5162-5171 [Abstract] [Full Text]  
  • Wang, I-C., Chen, Y.-J., Hughes, D. E., Ackerson, T., Major, M. L., Kalinichenko, V. V., Costa, R. H., Raychaudhuri, P., Tyner, A. L., Lau, L. F. (2008). FoxM1 Regulates Transcription of JNK1 to Promote the G1/S Transition and Tumor Cell Invasiveness. J. Biol. Chem. 283: 20770-20778 [Abstract] [Full Text]  
  • Kwok, J. M-M., Myatt, S. S., Marson, C. M., Coombes, R. C., Constantinidou, D., Lam, E. W-F. (2008). Thiostrepton selectively targets breast cancer cells through inhibition of forkhead box M1 expression. Molecular Cancer Therapeutics 7: 2022-2032 [Abstract] [Full Text]  
  • Calvisi, D. F., Pinna, F., Meloni, F., Ladu, S., Pellegrino, R., Sini, M., Daino, L., Simile, M. M., De Miglio, M. R., Virdis, P., Frau, M., Tomasi, M. L., Seddaiu, M. A., Muroni, M. R., Feo, F., Pascale, R. M. (2008). Dual-Specificity Phosphatase 1 Ubiquitination in Extracellular Signal-Regulated Kinase-Mediated Control of Growth in Human Hepatocellular Carcinoma. Cancer Res. 68: 4192-4200 [Abstract] [Full Text]  
  • Laoukili, J., Alvarez, M., Meijer, L. A. T., Stahl, M., Mohammed, S., Kleij, L., Heck, A. J. R., Medema, R. H. (2008). Activation of FoxM1 during G2 Requires Cyclin A/Cdk-Dependent Relief of Autorepression by the FoxM1 N-Terminal Domain. Mol. Cell. Biol. 28: 3076-3087 [Abstract] [Full Text]  
  • Petrovic, V., Costa, R. H., Lau, L. F., Raychaudhuri, P., Tyner, A. L. (2008). FoxM1 Regulates Growth Factor-induced Expression of Kinase-interacting Stathmin (KIS) to Promote Cell Cycle Progression. J. Biol. Chem. 283: 453-460 [Abstract] [Full Text]  
  • Masumoto, N., Tateno, C., Tachibana, A., Utoh, R., Morikawa, Y., Shimada, T., Momisako, H., Itamoto, T., Asahara, T., Yoshizato, K. (2007). GH enhances proliferation of human hepatocytes grafted into immunodeficient mice with damaged liver. J Endocrinol 194: 529-537 [Abstract] [Full Text]  
  • Freddie, C. T., Ji, Z., Marais, A., Sharrocks, A. D. (2007). Functional interactions between the Forkhead transcription factor FOXK1 and the MADS-box protein SRF. Nucleic Acids Res 0: gkm528v1-10 [Abstract] [Full Text]  
  • Tan, Y., Raychaudhuri, P., Costa, R. H. (2007). Chk2 Mediates Stabilization of the FoxM1 Transcription Factor To Stimulate Expression of DNA Repair Genes. Mol. Cell. Biol. 27: 1007-1016 [Abstract] [Full Text]  
  • Maeda, Y., Dave, V., Whitsett, J. A. (2007). Transcriptional Control of Lung Morphogenesis. Physiol. Rev. 87: 219-244 [Abstract] [Full Text]  
  • Chen, C., Rowell, E. A., Thomas, R. M., Hancock, W. W., Wells, A. D. (2006). Transcriptional Regulation by Foxp3 Is Associated with Direct Promoter Occupancy and Modulation of Histone Acetylation. J. Biol. Chem. 281: 36828-36834 [Abstract] [Full Text]  
  • Biggs, J. R., Peterson, L. F., Zhang, Y., Kraft, A. S., Zhang, D.-E. (2006). AML1/RUNX1 Phosphorylation by Cyclin-Dependent Kinases Regulates the Degradation of AML1/RUNX1 by the Anaphase-Promoting Complex. Mol. Cell. Biol. 26: 7420-7429 [Abstract] [Full Text]  
  • Radhakrishnan, S. K., Bhat, U. G., Hughes, D. E., Wang, I-C., Costa, R. H., Gartel, A. L. (2006). Identification of a Chemical Inhibitor of the Oncogenic Transcription Factor Forkhead Box M1. Cancer Res. 66: 9731-9735 [Abstract] [Full Text]  
  • Kim, I.-M., Ackerson, T., Ramakrishna, S., Tretiakova, M., Wang, I-C., Kalin, T. V., Major, M. L., Gusarova, G. A., Yoder, H. M., Costa, R. H., Kalinichenko, V. V. (2006). The Forkhead Box m1 Transcription Factor Stimulates the Proliferation of Tumor Cells during Development of Lung Cancer. Cancer Res. 66: 2153-2161 [Abstract] [Full Text]  
  • Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]  
  • Kalin, T. V., Wang, I-C., Ackerson, T. J., Major, M. L., Detrisac, C. J., Kalinichenko, V. V., Lyubimov, A., Costa, R. H. (2006). Increased Levels of the FoxM1 Transcription Factor Accelerate Development and Progression of Prostate Carcinomas in both TRAMP and LADY Transgenic Mice. Cancer Res. 66: 1712-1720 [Abstract] [Full Text]  
  • Wang, I-C., Chen, Y.-J., Hughes, D., Petrovic, V., Major, M. L., Park, H. J., Tan, Y., Ackerson, T., Costa, R. H. (2005). Forkhead Box M1 Regulates the Transcriptional Network of Genes Essential for Mitotic Progression and Genes Encoding the SCF (Skp2-Cks1) Ubiquitin Ligase. Mol. Cell. Biol. 25: 10875-10894 [Abstract] [Full Text]  
  • Lindqvist, A., Kallstrom, H., Lundgren, A., Barsoum, E., Rosenthal, C. K. (2005). Cdc25B cooperates with Cdc25A to induce mitosis but has a unique role in activating cyclin B1-Cdk1 at the centrosome. JCB 171: 35-45 [Abstract] [Full Text]  
  • Kim, I.-M., Ramakrishna, S., Gusarova, G. A., Yoder, H. M., Costa, R. H., Kalinichenko, V. V. (2005). The Forkhead Box M1 Transcription Factor Is Essential for Embryonic Development of Pulmonary Vasculature. J. Biol. Chem. 280: 22278-22286 [Abstract] [Full Text]  
  • Ma, R. Y. M., Tong, T. H. K., Cheung, A. M. S., Tsang, A. C. C., Leung, W. Y., Yao, K.-M. (2005). Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. J. Cell Sci. 118: 795-806 [Abstract] [Full Text]  
  • Foulds, C. E., Nelson, M. L., Blaszczak, A. G., Graves, B. J. (2004). Ras/Mitogen-Activated Protein Kinase Signaling Activates Ets-1 and Ets-2 by CBP/p300 Recruitment. Mol. Cell. Biol. 24: 10954-10964 [Abstract] [Full Text]  
  • Kalinichenko, V. V., Major, M. L., Wang, X., Petrovic, V., Kuechle, J., Yoder, H. M., Dennewitz, M. B., Shin, B., Datta, A., Raychaudhuri, P., Costa, R. H. (2004). Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor. Genes Dev. 18: 830-850 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»