This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Molinari, S.
Right arrow Articles by Buckingham, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Molinari, S.
Right arrow Articles by Buckingham, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2004, p. 2944-2957, Vol. 24, No. 7
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.7.2944-2957.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Novel Complex Regulates cardiac actin Gene Expression through Interaction of Emb, a Class VI POU Domain Protein, MEF2D, and the Histone Transacetylase p300

S. Molinari,1 F. Relaix,2 M. Lemonnier,2 B. Kirschbaum,3 B. Schäfer,4 and M. Buckingham2*

CNRS URA 2578, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France,2 Dipartimento di Scienze Biomediche, Università degli studi di Modena e Reggio Emilia, 41100 Modena, Italy,1 Aventis Pharma, D-65926 Frankfurt am Main, Germany,3 Department of Pediatrics, University of Zurich, CH-8032 Zurich, Switzerland4

Received 12 August 2002/ Returned for modification 23 October 2002/ Accepted 6 January 2004

Expression of the mouse cardiac actin gene depends on a distal enhancer (-7 kbp) which has been shown, in transgenic mice, to direct expression to embryonic skeletal muscle. The presence of this distal sequence is also associated with reproducible expression of cardiac actin transgenes. In differentiated skeletal muscle cells, activity of the enhancer is driven by an E box, binding MyoD family members, and by a 3' AT-rich sequence which is in the location of a DNase I-hypersensitive site. This sequence does not bind MEF2 proteins, or other known muscle transcription factors, directly. Oct1 and Emb, a class VI POU domain protein, bind to consensus sites on the DNA, and it is the binding of Emb which is important for activity. Emb binds as a major complex with MEF2D and the histone transacetylase p300. The form of Emb present in this complex and as a major form in muscle cell extracts is longer (80 kDa) than that previously described. These results demonstrate the importance of this novel complex in the transcriptional regulation of the cardiac actin gene and suggest a potential role in chromatin remodeling associated with muscle gene activation.

* Corresponding author. Mailing address: CNRS URA 1947, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 84 77. Fax: 33 1 40 61 34 52. E-mail: margab{at}pasteur.fr.

Molecular and Cellular Biology, April 2004, p. 2944-2957, Vol. 24, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.7.2944-2957.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Angelelli, C., Magli, A., Ferrari, D., Ganassi, M., Matafora, V., Parise, F., Razzini, G., Bachi, A., Ferrari, S., Molinari, S. (2008). Differentiation-dependent lysine 4 acetylation enhances MEF2C binding to DNA in skeletal muscle cells. Nucleic Acids Res 36: 915-928 [Abstract] [Full Text]  
  • Bajard, L., Relaix, F., Lagha, M., Rocancourt, D., Daubas, P., Buckingham, M. E. (2006). A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. Genes Dev. 20: 2450-2464 [Abstract] [Full Text]  
  • Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]  
  • Ma, K., Chan, J. K. L., Zhu, G., Wu, Z. (2005). Myocyte Enhancer Factor 2 Acetylation by p300 Enhances Its DNA Binding Activity, Transcriptional Activity, and Myogenic Differentiation. Mol. Cell. Biol. 25: 3575-3582 [Abstract] [Full Text]  
  • Lemonnier, M., Buckingham, M. E. (2004). Characterization of a Cardiac-specific Enhancer, Which Directs {alpha}-Cardiac Actin Gene Transcription in the Mouse Adult Heart. J. Biol. Chem. 279: 55651-55658 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»