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Molecular and Cellular Biology, April 2004, p. 2998-3010, Vol. 24, No. 7
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.7.2998-3010.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Loss of Translational Control in Yeast Compromised for the Major mRNA Decay Pathway

L. E. A. Holmes,1 S. G. Campbell,1 S. K. De Long,2 A. B. Sachs,2 and M. P. Ashe1*

Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester M60 1QD, United Kingdom,1 Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 947202

Received 8 October 2003/ Returned for modification 12 November 2003/ Accepted 15 January 2004

The cytoplasmic fate of mRNAs is dictated by the relative activities of the intimately connected mRNA decay and translation initiation pathways. In this study, we have found that yeast strains compromised for stages downstream of deadenylation in the major mRNA decay pathway are incapable of inhibiting global translation initiation in response to stress. In the past, the paradigm of the eIF2{alpha} kinase-dependent amino acid starvation pathway in yeast has been used to evaluate this highly conserved stress response in all eukaryotic cells. Using a similar approach we have found that even though the mRNA decay mutants maintain high levels of general translation, they exhibit many of the hallmarks of amino acid starvation, including increased eIF2{alpha} phosphorylation and activated GCN4 mRNA translation. Therefore, these mutants appear translationally oblivious to decreased ternary complex abundance, and we propose that this is due to higher rates of mRNA recruitment to the 40S ribosomal subunit.

* Corresponding author. Mailing address: Department of Biomolecular Sciences, UMIST, Sackville St., P.O. Box 88, Manchester M60 1QD, United Kingdom. Phone: 44 161 200 4164. Fax: 44 161 200 0409. E-mail: mark.p.ashe{at}umist.ac.uk.

Molecular and Cellular Biology, April 2004, p. 2998-3010, Vol. 24, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.7.2998-3010.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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