This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomita, A.
Right arrow Articles by Shi, Y.-B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomita, A.
Right arrow Articles by Shi, Y.-B.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2004, p. 3337-3346, Vol. 24, No. 8
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.8.3337-3346.2004

Recruitment of N-CoR/SMRT-TBLR1 Corepressor Complex by Unliganded Thyroid Hormone Receptor for Gene Repression during Frog Development

Akihiro Tomita, Daniel R. Buchholz, and Yun-Bo Shi*

Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-5431

Received 10 November 2003/ Returned for modification 9 December 2003/ Accepted 27 January 2004

The corepressors N-CoR (nuclear receptor corepressor) and SMRT (silencing mediator for retinoid and thyroid hormone receptors) interact with unliganded nuclear hormone receptors, including thyroid hormone (T3) receptor (TR). Several N-CoR/SMRT complexes containing histone deacetylases have been purified. The best studied among them are N-CoR/SMRT complexes containing TBL1 (transducin beta-like protein 1) or TBLR1 (TBL1-related protein). Despite extensive studies of these complexes, there has been no direct in vivo evidence for the interaction of TBL1 or TBLR1 with TR or the possible involvement of such complexes in gene repression by any nuclear receptors in any animals. Here, we used the frog oocyte system to demonstrate that unliganded TR interacts with TBLR1 and recruits TBLR1 to its chromatinized target promoter in vivo, accompanied by histone deacetylation and gene repression. We further provide evidence to show that the recruitment of TBLR1 or related proteins is important for repression by unliganded TR. To investigate the potential role for TBLR1 complexes during vertebrate development, we made use of T3-dependent amphibian metamorphosis as a model. We found that TBLR1, SMRT, and N-CoR are recruited to T3-inducible promoters in premetamorphic tadpoles and are released upon T3 treatment, which induces metamorphosis. More importantly, we demonstrate that the dissociation of N-CoR/SMRT-TBLR1 complexes from endogenous TR target promoters is correlated with the activation of these genes during spontaneous metamorphosis. Taken together, our studies provide in vivo evidence for targeted recruitment of N-CoR/SMRT-TBLR1 complexes by unliganded TR in transcriptional repression during vertebrate development.

* Corresponding author. Mailing address: Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, NICHD/NIH, Building 18T, Room 106, Bethesda, MD 20892-5431. Phone: (301) 402-1004. Fax: (301) 402-1323. E-mail: shi{at}helix.nih.gov.

Molecular and Cellular Biology, April 2004, p. 3337-3346, Vol. 24, No. 8
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.8.3337-3346.2004




This article has been cited by other articles:

  • Heimeier, R. A., Das, B., Buchholz, D. R., Shi, Y.-B. (2009). The Xenoestrogen Bisphenol A Inhibits Postembryonic Vertebrate Development by Antagonizing Gene Regulation by Thyroid Hormone. Endocrinology 150: 2964-2973 [Abstract] [Full Text]  
  • Wang, D., Xia, X., Liu, Y., Oetting, A., Walker, R. L., Zhu, Y., Meltzer, P., Cole, P. A., Shi, Y.-B., Yen, P. M. (2009). Negative Regulation of TSH{alpha} Target Gene by Thyroid Hormone Involves Histone Acetylation and Corepressor Complex Dissociation. Mol. Endocrinol. 23: 600-609 [Abstract] [Full Text]  
  • Matsuda, H., Paul, B. D., Choi, C. Y., Hasebe, T., Shi, Y.-B. (2009). Novel Functions of Protein Arginine Methyltransferase 1 in Thyroid Hormone Receptor-Mediated Transcription and in the Regulation of Metamorphic Rate in Xenopus laevis. Mol. Cell. Biol. 29: 745-757 [Abstract] [Full Text]  
  • Wang, X., Matsuda, H., Shi, Y.-B. (2008). Developmental Regulation and Function of Thyroid Hormone Receptors and 9-cis Retinoic Acid Receptors during Xenopus tropicalis Metamorphosis. Endocrinology 149: 5610-5618 [Abstract] [Full Text]  
  • Heimeier, R. A., Hsia, V. S., Shi, Y.-B. (2008). Participation of Brahma-Related Gene 1 (BRG1)-Associated Factor 57 and BRG1-Containing Chromatin Remodeling Complexes in Thyroid Hormone-Dependent Gene Activation during Vertebrate Development. Mol. Endocrinol. 22: 1065-1077 [Abstract] [Full Text]  
  • Matsuda, H., Paul, B. D., Choi, C. Y., Shi, Y.-B. (2007). Contrasting Effects of Two Alternative Splicing Forms of Coactivator-Associated Arginine Methyltransferase 1 on Thyroid Hormone Receptor-Mediated Transcription in Xenopus laevis. Mol. Endocrinol. 21: 1082-1094 [Abstract] [Full Text]  
  • Paul, B. D., Buchholz, D. R., Fu, L., Shi, Y.-B. (2007). SRC-p300 Coactivator Complex Is Required for Thyroid Hormone-induced Amphibian Metamorphosis. J. Biol. Chem. 282: 7472-7481 [Abstract] [Full Text]  
  • Gropp, F. N. C., Greger, D. L., Morel, C., Sauter, S., Blum, J. W. (2006). Nuclear receptor and nuclear receptor target gene messenger ribonucleic acid levels at different sites of the gastrointestinal tract and in liver of healthy dogs. J ANIM SCI 84: 2684-2691 [Abstract] [Full Text]  
  • Fu, L., Tomita, A., Wang, H., Buchholz, D. R., Shi, Y.-B. (2006). Transcriptional Regulation of the Xenopus laevis Stromelysin-3 Gene by Thyroid Hormone Is Mediated by a DNA Element in the First Intron. J. Biol. Chem. 281: 16870-16878 [Abstract] [Full Text]  
  • Rosenfeld, M. G., Lunyak, V. V., Glass, C. K. (2006). Sensors and signals: a coactivator/corepressor/epigenetic code for integrating signal-dependent programs of transcriptional response. Genes Dev. 20: 1405-1428 [Abstract] [Full Text]  
  • Clem, B. F., Clark, B. J. (2006). Association of the mSin3A-Histone Deacetylase 1/2 Corepressor Complex with the Mouse Steroidogenic Acute Regulatory Protein Gene. Mol. Endocrinol. 20: 100-113 [Abstract] [Full Text]  
  • Buchholz, D. R., Paul, B. D., Shi, Y.-B. (2005). Gene-specific Changes in Promoter Occupancy by Thyroid Hormone Receptor during Frog Metamorphosis: IMPLICATIONS FOR DEVELOPMENTAL GENE REGULATION. J. Biol. Chem. 280: 41222-41228 [Abstract] [Full Text]  
  • Paul, B. D., Buchholz, D. R., Fu, L., Shi, Y.-B. (2005). Tissue- and Gene-specific Recruitment of Steroid Receptor Coactivator-3 by Thyroid Hormone Receptor during Development. J. Biol. Chem. 280: 27165-27172 [Abstract] [Full Text]  
  • Paul, B. D., Fu, L., Buchholz, D. R., Shi, Y.-B. (2005). Coactivator Recruitment Is Essential for Liganded Thyroid Hormone Receptor To Initiate Amphibian Metamorphosis. Mol. Cell. Biol. 25: 5712-5724 [Abstract] [Full Text]  
  • Codina, A., Love, J. D., Li, Y., Lazar, M. A., Neuhaus, D., Schwabe, J. W. R. (2005). From The Cover: Structural insights into the interaction and activation of histone deacetylase 3 by nuclear receptor corepressors. Proc. Natl. Acad. Sci. USA 102: 6009-6014 [Abstract] [Full Text]  
  • Peng, G.-H., Ahmad, O., Ahmad, F., Liu, J., Chen, S. (2005). The photoreceptor-specific nuclear receptor Nr2e3 interacts with Crx and exerts opposing effects on the transcription of rod versus cone genes. Hum Mol Genet 14: 747-764 [Abstract] [Full Text]  
  • Olson, L. E., Zhang, J., Taylor, H., Rose, D. W., Rosenfeld, M. G. (2005). Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling. Proc. Natl. Acad. Sci. USA 102: 3708-3713 [Abstract] [Full Text]  
  • Goodson, M. L., Jonas, B. A., Privalsky, M. L. (2005). Alternative mRNA Splicing of SMRT Creates Functional Diversity by Generating Corepressor Isoforms with Different Affinities for Different Nuclear Receptors. J. Biol. Chem. 280: 7493-7503 [Abstract] [Full Text]  
  • Buchholz, D. R., Tomita, A., Fu, L., Paul, B. D., Shi, Y.-B. (2004). Transgenic Analysis Reveals that Thyroid Hormone Receptor Is Sufficient To Mediate the Thyroid Hormone Signal in Frog Metamorphosis. Mol. Cell. Biol. 24: 9026-9037 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»