Previous Article | Next Article 
Molecular and Cellular Biology, April 2004, p. 3347-3358, Vol. 24, No. 8
0270-7306/04/$08.00+0 DOI: 10.1128/MCB.24.8.3347-3358.2004
Reconstitution of Glucocorticoid Receptor-Dependent Transcription In Vivo
Kevin W. Trotter and Trevor K. Archer*
Chromatin and Gene Expression Section, Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709
Received 2 December 2003/
Returned for modification 5 January 2004/
Accepted 27 January 2004
We developed a model system to study glucocorticoid receptor (GR)-mediated chromatin remodeling by the BRG1 complex. Introduction of the BRG1 ATPase into the SW-13 cell line initiates the formation of a functional remodeling complex. This complex is able to induce transcriptional activation from a transiently transfected promoter with wild-type and chromatin-remodeling-deficient BRG1 mutants, suggesting that the complex possesses a coactivator function independent from remodeling. Transactivation from a chromatin template requires the BRG1 remodeling function, which induces regions of hypersensitivity and transcription factor loading onto the integrated MMTV promoter. We report that BRG1 remodeling activity is required for GR-mediated transactivation and that this activity cannot be replaced by other ATP-dependent remodeling proteins. Further characterization of the BRG1-associated factors (BAFs) present in these cells (for example, the expression of BAF250 but not BAF180) reveals that the BAF complex rather than the polybromo-associated BAF complex is the necessary and sufficient chromatin-remodeling component with which the receptor functions in vivo. These results in conjunction with previous findings demonstrate that the GR functions with multiple forms of the SWI/SNF complex in vivo.
* Corresponding author. Mailing address: Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T. W. Alexander Dr., P. O. Box 12233 (MD E4-06), Research Triangle Park, NC 27709. Phone: (919) 316-4565. Fax: (919) 316-4566. E-mail: archer1{at}niehs.nih.gov.
Molecular and Cellular Biology, April 2004, p. 3347-3358, Vol. 24, No. 8
0022-538X/04/$08.00+0 DOI: 10.1128/MCB.24.8.3347-3358.2004
This article has been cited by other articles:
-
Carrera, I., Zavadil, J., Treisman, J. E.
(2008). Two Subunits Specific to the PBAP Chromatin Remodeling Complex Have Distinct and Redundant Functions during Drosophila Development. Mol. Cell. Biol.
28: 5238-5250
[Abstract]
[Full Text]
-
Oh, J., Sohn, D. H., Ko, M., Chung, H., Jeon, S. H., Seong, R. H.
(2008). BAF60a Interacts with p53 to Recruit the SWI/SNF Complex. J. Biol. Chem.
283: 11924-11934
[Abstract]
[Full Text]
-
Hebbar, P. B., Archer, T. K.
(2008). Altered Histone H1 Stoichiometry and an Absence of Nucleosome Positioning on Transfected DNA. J. Biol. Chem.
283: 4595-4601
[Abstract]
[Full Text]
-
Trotter, K. W., Fan, H.-Y., Ivey, M. L., Kingston, R. E., Archer, T. K.
(2008). The HSA Domain of BRG1 Mediates Critical Interactions Required for Glucocorticoid Receptor-Dependent Transcriptional Activation In Vivo. Mol. Cell. Biol.
28: 1413-1426
[Abstract]
[Full Text]
-
Aoyagi, S., Archer, T. K.
(2008). Nicotinamide Uncouples Hormone-Dependent Chromatin Remodeling from Transcription Complex Assembly. Mol. Cell. Biol.
28: 30-39
[Abstract]
[Full Text]
-
Kinyamu, H. K., Archer, T. K.
(2007). Proteasome Activity Modulates Chromatin Modifications and RNA Polymerase II Phosphorylation To Enhance Glucocorticoid Receptor-Mediated Transcription. Mol. Cell. Biol.
27: 4891-4904
[Abstract]
[Full Text]
-
Lee, K. Y., Choi, Y. I., Kim, J., Choi, J. W., Sohn, D. H., Lee, C., Jeon, S. H., Seong, R. H.
(2007). Down-Regulation of the SWI/SNF Chromatin Remodeling Activity by TCR Signaling Is Required for Proper Thymocyte Maturation. J. Immunol.
178: 7088-7096
[Abstract]
[Full Text]
-
Sohn, D. H., Lee, K. Y., Lee, C., Oh, J., Chung, H., Jeon, S. H., Seong, R. H.
(2007). SRG3 Interacts Directly with the Major Components of the SWI/SNF Chromatin Remodeling Complex and Protects Them from Proteasomal Degradation. J. Biol. Chem.
282: 10614-10624
[Abstract]
[Full Text]
-
Hebbar, P. B., Archer, T. K.
(2007). Chromatin-dependent Cooperativity between Site-specific Transcription Factors in Vivo. J. Biol. Chem.
282: 8284-8291
[Abstract]
[Full Text]
-
Burkhart, B. A., Kennett, S. B., Archer, T. K.
(2007). Osmotic Stress-dependent Repression Is Mediated by Histone H3 Phosphorylation and Chromatin Structure. J. Biol. Chem.
282: 4400-4407
[Abstract]
[Full Text]
-
Moshkin, Y. M., Mohrmann, L., van Ijcken, W. F. J., Verrijzer, C. P.
(2007). Functional Differentiation of SWI/SNF Remodelers in Transcription and Cell Cycle Control. Mol. Cell. Biol.
27: 651-661
[Abstract]
[Full Text]
-
Chen, J., Kinyamu, H. K., Archer, T. K.
(2006). Changes in Attitude, Changes in Latitude: Nuclear Receptors Remodeling Chromatin to Regulate Transcription. Mol. Endocrinol.
20: 1-13
[Abstract]
[Full Text]
-
Baserga, M., Hale, M. A., McKnight, R. A., Yu, X., Callaway, C. W., Lane, R. H.
(2005). Uteroplacental insufficiency alters hepatic expression, phosphorylation, and activity of the glucocorticoid receptor in fetal IUGR rats. Am. J. Physiol. Regul. Integr. Comp. Physiol.
289: R1348-R1353
[Abstract]
[Full Text]
-
Chen, J., Archer, T. K.
(2005). Regulating SWI/SNF Subunit Levels via Protein-Protein Interactions and Proteasomal Degradation: BAF155 and BAF170 Limit Expression of BAF57. Mol. Cell. Biol.
25: 9016-9027
[Abstract]
[Full Text]
-
Huuskonen, J., Vishnu, M., Fielding, P. E., Fielding, C. J.
(2005). Activation of ATP-Binding Cassette Transporter A1 Transcription by Chromatin Remodeling Complex. Arterioscler. Thromb. Vasc. Bio.
25: 1180-1185
[Abstract]
[Full Text]
-
Burkhart, B. A., Hebbar, P. B., Trotter, K. W., Archer, T. K.
(2005). Chromatin-dependent E1A Activity Modulates NF-{kappa}B RelA-mediated Repression of Glucocorticoid Receptor-dependent Transcription. J. Biol. Chem.
280: 6349-6358
[Abstract]
[Full Text]
Copyright © 2004 by the American Society for Microbiology. All rights reserved.