This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koloteva-Levine, N.
Right arrow Articles by Elroy-Stein, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koloteva-Levine, N.
Right arrow Articles by Elroy-Stein, O.

Next Article 

Molecular and Cellular Biology, May 2004, p. 3577-3587, Vol. 24, No. 9
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.9.3577-3587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses Internal Ribosome Entry Site-Mediated Translation

Nadejda Koloteva-Levine,1,{dagger} Dalia Pinchasi,1 Idan Pereman,1 Amit Zur,2 Michael Brandeis,2 and Orna Elroy-Stein1*

Department of Cell Research & Immunology, George S. Wise Faculty of Life Science, Tel Aviv University, Tel Aviv,1 Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel2

Received 7 October 2003/ Returned for modification 20 November 2003/ Accepted 9 February 2004

The anaphase-promoting complex/cyclosome (APC/C) is a multisubunit ubiquitin ligase that mediates the proteolysis of cell cycle proteins in mitosis and G1. We used a yeast three-hybrid screen to identify proteins that interact with the internal ribosome entry site (IRES) of platelet-derived growth factor 2 mRNA. Surprisingly, this screen identified Apc5, although it does not harbor a classical RNA binding domain. We found that Apc5 binds the poly(A) binding protein (PABP), which directly binds the IRES element. PABP was found to enhance IRES-mediated translation, whereas Apc5 overexpression counteracted this effect. In addition to its association with the APC/C complex, Apc5 binds much heavier complexes and cosediments with the ribosomal fraction. In contrast to Apc3, which is associated only with the APC/C and remains intact during differentiation, Apc5 is degraded upon megakaryocytic differentiation in correlation with IRES activation. Expression of Apc5 in differentiated cells abolished IRES activation. This is the first report implying an additional role for an APC/C subunit, apart from its being part of the APC/C complex.

* Corresponding author. Mailing address: Department of Cell Research & Immunology, George S. Wise Faculty of Life Science, Tel Aviv University, Tel Aviv 69978, Israel. Phone: 972-3-640-9153. Fax: 972-3-642-2046. E-mail: ornaes{at}post.tau.ac.il.

{dagger} Present address: Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom.

Molecular and Cellular Biology, May 2004, p. 3577-3587, Vol. 24, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.9.3577-3587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dizin, E., Gressier, C., Magnard, C., Ray, H., Decimo, D., Ohlmann, T., Venezia, N. D. (2006). BRCA1 Interacts with Poly(A)-binding Protein: IMPLICATION OF BRCA1 IN TRANSLATION REGULATION. J. Biol. Chem. 281: 24236-24246 [Abstract] [Full Text]  
  • Kozak, M. (2005). A second look at cellular mRNA sequences said to function as internal ribosome entry sites. Nucleic Acids Res 33: 6593-6602 [Abstract] [Full Text]  
  • Cho, S., Kim, J. H., Back, S. H., Jang, S. K. (2005). Polypyrimidine Tract-Binding Protein Enhances the Internal Ribosomal Entry Site-Dependent Translation of p27Kip1 mRNA and Modulates Transition from G1 to S Phase. Mol. Cell. Biol. 25: 1283-1297 [Abstract] [Full Text]  
  • Arnason, T. G., Pisclevich, M. G., Dash, M. D., Davies, G. F., Harkness, T. A. A. (2005). Novel Interaction between Apc5p and Rsp5p in an Intracellular Signaling Pathway in Saccharomyces cerevisiae. Eukaryot Cell 4: 134-146 [Abstract] [Full Text]  
  • Harkness, T. A. A., Shea, K. A., Legrand, C., Brahmania, M., Davies, G. F. (2004). A Functional Analysis Reveals Dependence on the Anaphase-Promoting Complex for Prolonged Life Span in Yeast. Genetics 168: 759-774 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»