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Molecular and Cellular Biology, May 2004, p. 4075-4082, Vol. 24, No. 9
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.9.4075-4082.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Targeted Disruption of SPI3/Serpinb6 Does Not Result in Developmental or Growth Defects, Leukocyte Dysfunction, or Susceptibility to Stroke

Katrina L. Scarff,¹ Kheng S. Ung,¹ Harshal Nandurkar,² Peter J. Crack,³ Catherina H. Bird,¹ and Phillip I. Bird^1*

Department of Biochemistry and Molecular Biology,¹ Centre for Functional Genomics and Human Disease, Monash Institute of Reproduction and Development, Monash University, Clayton, Victoria 3800,³ St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia²

Received 26 November 2003/ Returned for modification 24 January 2004/ Accepted 2 February 2004

Protease inhibitor 6 (PI-6/SERPINB6) is a widely expressed nucleocytoplasmic serpin. It inhibits granulocyte cathepsin G and neuronal neuropsin, and it is thought to protect cells from death caused by ectopic release or internalization of protease during stress such as infection or cerebral ischemia. To probe the biological functions of PI-6, we generated mice lacking its ortholog (SPI3/Serpinb6). SPI3-deficient mice developed normally and were fertile, and no abnormal pathology or increased sensitivity to cerebral ischemia was observed. There were no perturbations in leukocyte development or numbers, and recruitment of leukocytes to the peritoneal cavity was normal. SPI3-deficient mice were equally susceptible as wild-type mice to systemic Candida albicans infection, although there was a slight decrease in the ability of neutrophils from SPI3-deficient mice to kill C. albicans in vitro. Increased levels of a related inhibitor Serpinb1 (monocyte/neutrophil elastase inhibitor) in the tissues of targeted mice suggests that compensation by other serpins reduces the impact of SPI3 deficiency in these animals and may explain the lack of a more obvious phenotype.

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* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Monash University, Wellington Rd., Clayton, Victoria 3800, Australia. Phone: 61 3 9905 3771. Fax: 61 3 9905 3726. E-mail: phil.bird{at}med.monash.edu.au.

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Molecular and Cellular Biology, May 2004, p. 4075-4082, Vol. 24, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.9.4075-4082.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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