This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Holmes, A. M. Articles by Arcangioli, B. Search for Related Content PubMed PubMed Citation Articles by Holmes, A. M. Articles by Arcangioli, B.

Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe

Unité de Dynamique du Génome, URA 1644 du CNRS, Departement de la Structure et Dynamique des Génomes, Institut Pasteur, Paris, France

Received 26 July 2004/ Returned for modification 20 September 2004/ Accepted 4 October 2004

A strand-specific imprint (break) controls mating-type switching in fission yeast. By introducing a thiamine repressible promoter upstream of the mat1 locus, we can force transcription through the imprinted region, erasing the imprint and inhibiting further mating-type switching, in a reversible manner. Starting from a synchronized, virgin M-cell population, we show that the site- and strand-specific break is formed when DNA replication intermediates appear at mat1 during the first S phase. The formation of the break is concomitant with a replication fork pause and binding of the Swi1 protein at mat1 until early G₂ and then rapidly disappears. Upon its formation, the break remains stable throughout the cell cycle and triggers mating-type switching during the second S phase. Finally, we have recreated the mating-type switching pedigree at the molecular and single-cell levels, allowing for the first time separation between the establishment of imprinting and its developmental fate.

This article has been cited by other articles:

• Yamada-Inagawa, T., Klar, A. J. S., Dalgaard, J. Z. (2007). Schizosaccharomyces pombe Switches Mating Type by the Synthesis-Dependent Strand-Annealing Mechanism. Genetics 177: 255-265 [Abstract] [Full Text]  
• Xhemalce, B., Miller, K. M., Driscoll, R., Masumoto, H., Jackson, S. P., Kouzarides, T., Verreault, A., Arcangioli, B. (2007). Regulation of Histone H3 Lysine 56 Acetylation in Schizosaccharomyces pombe. J. Biol. Chem. 282: 15040-15047 [Abstract] [Full Text]  
• Ira, G., Satory, D., Haber, J. E. (2006). Conservative Inheritance of Newly Synthesized DNA in Double-Strand Break-Induced Gene Conversion. Mol. Cell. Biol. 26: 9424-9429 [Abstract] [Full Text]  
• Nedelcheva-Veleva, M. N., Krastev, D. B., Stoynov, S. S. (2006). Coordination of DNA synthesis and replicative unwinding by the S-phase checkpoint pathways. Nucleic Acids Res 34: 4138-4146 [Abstract] [Full Text]