This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hiratsuka, S.
Right arrow Articles by Shibuya, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hiratsuka, S.
Right arrow Articles by Shibuya, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2005, p. 355-363, Vol. 25, No. 1
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.1.355-363.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Vascular Endothelial Growth Factor A (VEGF-A) Is Involved in Guidance of VEGF Receptor-Positive Cells to the Anterior Portion of Early Embryos

Sachie Hiratsuka,1,2 Yuki Kataoka,3 Kazuki Nakao,4 Kenji Nakamura,5 Shunichi Morikawa,6 Satoshi Tanaka,7 Motoya Katsuki,8 Yoshiro Maru,2 and Masabumi Shibuya1*

Department of Genetics,1 Laboratory of Gene Expression and Regulation, Institute of Medical Science,3 Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Science, University of Tokyo,7 Department of Pharmacology,2 Department of Anatomy & Developmental Biology, Tokyo Women's Medical University School of Medicine,6 Reproductive Engineering Section, Mouse Genome Technology Center, Mitsubishi Kagaku Institute of Life Science, Tokyo,5 Laboratory for Animal Resource and Genetic Engineering, RIKEN, Center for Developmental Biology, Hyogo,4 National Institute for Basic Biology, Aichi, Japan8

Received 27 June 2004/ Returned for modification 4 August 2004/ Accepted 4 October 2004

The hemangioblast in the mesoderm gives rise to both angioblasts and hematopoietic stem cells. The movement of hemangioblast precursor cells in the fetal trunk is a critical event in early embryogenesis. Vascular endothelial growth factor (VEGF) signaling is likely involved in this migration given the partial disturbance of VEGF receptor (VEGFR)-positive cell accumulation and migration in VEGFR2 null mice or mice with a truncated VEGFR1. However, it is not clear how the VEGF system regulates this migration or its direction. We show here that the expression of VEGF-A is dominant in the anterior portion of the embryo, whereas VEGFR1 and VEGFR2 are expressed in the posterior portion of the embryo. An inhibitor of VEGFR kinase blocked the migration of VEGFR-positive cells in a whole-embryo culture system. In addition, VEGFR-positive cells migrated toward a VEGFR1- or VEGFR2-specific ligand in vitro. Furthermore, VEGFR-positive cells derived from wild-type or VEGFR2+/– mice moved rapidly anteriorly, whereas cells derived from VEGFR2+/– mice carrying a truncated VEGFR1 [VEGFR1(TM-TK)–/–] migrated little when injected into wild-type mice. These results suggest that the VEGF-A protein concentrated in the anterior region plays an important role in the guidance of VEGFR-positive cells from the posterior portion to the head region by interacting with VEGFR in the mouse embryo.

* Corresponding author. Mailing address: Department of Genetics, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan. Phone: 81-3-5449-5550. Fax: 81-3-5449-5425. E-mail: shibuya{at}ims.u-tokyo.ac.jp.

Molecular and Cellular Biology, January 2005, p. 355-363, Vol. 25, No. 1
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.1.355-363.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guo, Q., Carrero, J. J., Yu, X., Barany, P., Qureshi, A. R., Eriksson, M., Anderstam, B., Chmielewski, M., Heimburger, O., Stenvinkel, P., Lindholm, B., Axelsson, J. (2009). Associations of VEGF and its receptors sVEGFR-1 and -2 with cardiovascular disease and survival in prevalent haemodialysis patients. Nephrol Dial Transplant 0: gfp315v1-gfp315 [Abstract] [Full Text]  
  • Saint-Geniez, M., Kurihara, T., D'Amore, P. A. (2009). Role of Cell and Matrix-Bound VEGF Isoforms in Lens Development. IOVS 50: 311-321 [Abstract] [Full Text]  
  • Janer, J., Andersson, S., Haglund, C., Karikoski, R., Lassus, P. (2008). Placental Growth Factor and Vascular Endothelial Growth Factor Receptor-2 in Human Lung Development. Pediatrics 122: 340-346 [Abstract] [Full Text]  
  • Kawasaki, K., Watabe, T., Sase, H., Hirashima, M., Koide, H., Morishita, Y., Yuki, K., Sasaoka, T., Suda, T., Katsuki, M., Miyazono, K., Miyazawa, K. (2008). Ras signaling directs endothelial specification of VEGFR2+ vascular progenitor cells. JCB 181: 131-141 [Abstract] [Full Text]  
  • Bahary, N., Goishi, K., Stuckenholz, C., Weber, G., LeBlanc, J., Schafer, C. A., Berman, S. S., Klagsbrun, M., Zon, L. I. (2007). Duplicate VegfA genes and orthologues of the KDR receptor tyrosine kinase family mediate vascular development in the zebrafish. Blood 110: 3627-3636 [Abstract] [Full Text]  
  • Schmidt, A., Brixius, K., Bloch, W. (2007). Endothelial Precursor Cell Migration During Vasculogenesis. Circ. Res. 101: 125-136 [Abstract] [Full Text]  
  • Duloquin, L., Lhomond, G., Gache, C. (2007). Localized VEGF signaling from ectoderm to mesenchyme cells controls morphogenesis of the sea urchin embryo skeleton. Development 134: 2293-2302 [Abstract] [Full Text]  
  • Tammela, T., Enholm, B., Alitalo, K., Paavonen, K. (2005). The biology of vascular endothelial growth factors. Cardiovasc Res 65: 550-563 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»