This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dou, Y.
Right arrow Articles by Gorovsky, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dou, Y.
Right arrow Articles by Gorovsky, M. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2005, p. 3914-3922, Vol. 25, No. 10
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.10.3914-3922.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The H1 Phosphorylation State Regulates Expression of CDC2 and Other Genes in Response to Starvation in Tetrahymena thermophila

Yali Dou ,{dagger},{ddagger} Xiaoyuan Song,{ddagger} Yifan Liu,{dagger} and Martin A. Gorovsky*

Department of Biology, University of Rochester, Rochester, New York 14627

Received 18 November 2004/ Returned for modification 13 January 2005/ Accepted 16 February 2005

In Tetrahymena thermophila, highly phosphorylated histone H1 of growing cells becomes partially dephosphorylated when cells are starved in preparation for conjugation. To determine the effects of H1 phosphorylation on gene expression, PCR-based subtractive hybridization was used to clone cDNAs that were differentially expressed during starvation in two otherwise-isogenic strains differing only in their H1s. H1 in A5 mutant cells lacked phosphorylation, and H1 in E5 cells mimicked constitutive H1 phosphorylation. Sequences enriched in A5 cells included genes encoding proteases. Sequences enriched in E5 cells included genes encoding cdc2 kinase and a Ser/Thr kinase. These results indicate that H1 phosphorylation plays an important role in regulating the pattern of gene expression during the starvation response and that its role in transcription regulation can be either positive or negative. Treatment of starved cells with a phosphatase inhibitor caused CDC2 gene overexpression. Expression of the E5 version of H1 in starved cells containing endogenous, wild-type H1 caused the wild-type H1 to remain highly phosphorylated. These results argue that Cdc2p is the kinase that phosphorylates Tetrahymena H1, establish a positive feedback mechanism between H1 phosphorylation and CDC2 expression, and indicate that CDC2 gene expression is regulated by an H1 phosphatase.

* Corresponding author. Mailing address: Department of Biology, University of Rochester, Rochester, NY 14627. Phone: (585) 275-6988. Fax: (585) 275-2070. E-mail: goro{at}mail.rochester.edu.

{dagger} Present address: The Rockefeller University, New York, NY 10021.

{ddagger} Y.D. and X.S. were equal contributors to this paper.

Molecular and Cellular Biology, May 2005, p. 3914-3922, Vol. 25, No. 10
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.10.3914-3922.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nock, A., Ascano, J. M., Jones, T., Barrero, M. J., Sugiyama, N., Tomita, M., Ishihama, Y., Malik, S. (2009). Identification of DNA-dependent Protein Kinase as a Cofactor for the Forkhead Transcription Factor FoxA2. J. Biol. Chem. 284: 19915-19926 [Abstract] [Full Text]  
  • Tsao, C.-C., Gorovsky, M. A. (2008). Different Effects of Tetrahymena IFT172 Domains on Anterograde and Retrograde Intraflagellar Transport. Mol. Biol. Cell 19: 1450-1461 [Abstract] [Full Text]  
  • Tsao, C.-C., Gorovsky, M. A. (2008). Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body. J. Cell Sci. 121: 428-436 [Abstract] [Full Text]  
  • Parker, K., Maxson, J., Mooney, A., Wiley, E. A. (2007). Class I Histone Deacetylase Thd1p Promotes Global Chromatin Condensation in Tetrahymena thermophila. Eukaryot Cell 6: 1913-1924 [Abstract] [Full Text]  
  • Song, X., Gorovsky, M. A. (2007). Unphosphorylated H1 Is Enriched in a Specific Region of the Promoter when CDC2 Is Down-Regulated during Starvation. Mol. Cell. Biol. 27: 1925-1933 [Abstract] [Full Text]  
  • Garcia, B. A., Joshi, S., Thomas, C. E., Chitta, R. K., Diaz, R. L., Busby, S. A., Andrews, P. C., Ogorzalek Loo, R. R., Shabanowitz, J., Kelleher, N. L., Mizzen, C. A., Allis, C. D., Hunt, D. F. (2006). Comprehensive Phosphoprotein Analysis of Linker Histone H1 from Tetrahymena thermophila. Mol. Cell. Proteomics 5: 1593-1609 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»