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Molecular and Cellular Biology, May 2005, p. 3934-3944, Vol. 25, No. 10
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.10.3934-3944.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-Strand Breaks{dagger}

Eun Yong Shim,{ddagger} Jia-Lin Ma,{ddagger} Ji-Hyun Oum,{ddagger} Yvonne Yanez, and Sang Eun Lee*

Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245

Received 3 December 2004/ Returned for modification 27 December 2004/ Accepted 9 February 2005

Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30{Delta} maintains a robust level of homologous recombination and the damage-induced cell cycle checkpoints. By chromatin immunoprecipitation, we show recruitment of RSC to a chromosome DSB with kinetics congruent with its involvement in NHEJ. Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p. The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ.

* Corresponding author. Mailing address: Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245. Phone: (210) 567-7273. Fax: (210) 567-7269. E-mail: lees4{at}uthscsa.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Eun Yong Shim, Jia-Lin Ma, and Ji-Hyun Oum are co-first authors.

Molecular and Cellular Biology, May 2005, p. 3934-3944, Vol. 25, No. 10
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.10.3934-3944.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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