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Mouse Axin and Axin2/Conductin Proteins Are Functionally Equivalent In Vivo

Department of Genetics and Development, Columbia University Medical Center, 701 W. 168th Street, New York, New York 10032

Received 18 January 2005/ Returned for modification 24 February 2005/ Accepted 2 March 2005

Axin is a central component of the canonical Wnt signal transduction machinery, serving as a scaffold for the ß-catenin destruction complex. The related protein Axin2/Conductin, although less extensively studied, is thought to perform similar functions. Loss of Axin causes early embryonic lethality, while Axin2-null mice are viable but have craniofacial defects. Mutations in either gene contribute to cancer in humans. The lack of redundancy between Axin and Axin2 could be due to their different modes of expression: while Axin is expressed ubiquitously, Axin2 is expressed in tissue- and developmental-stage-specific patterns, and its transcription is induced by canonical Wnt signaling. Alternatively, the two proteins might have partially different functions, a hypothesis supported by the observation that they differ in their subcellular localizations in colon epithelial cells. To test the functional equivalence of Axin and Axin2 in vivo, we generated knockin mice in which the Axin gene was replaced with Myc-tagged Axin or Axin2 cDNA. Mice homozygous for the resulting alleles, Axin^Ax or Axin^Ax2, express no endogenous Axin but express either Myc-Axin or Myc-Axin2 under the control of the Axin locus. Both Axin^Ax/Ax and Axin^Ax2/Ax2 homozygotes are apparently normal and fertile, demonstrating that the Axin and Axin2 proteins are functionally equivalent.

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