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Molecular and Cellular Biology, June 2005, p. 4541-4551, Vol. 25, No. 11
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.11.4541-4551.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Dual-Specificity Phosphatase CDC14B Bundles and Stabilizes Microtubules{dagger}

Hyekyung P. Cho,1 Yie Liu,1 Marla Gomez,1 John Dunlap,2 Mike Tyers,3 and Yisong Wang1*

Life Sciences Division, Oak Ridge National Laboratory, Bethel Valley Road, Oak Ridge, Tennessee 37831,1 Microscopy Facility, Division of Biology, University of Tennessee, 1414 W. Cumberland Avenue, Knoxville, Tennessee 37996,2 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada3

Received 17 August 2004/ Returned for modification 6 October 2004/ Accepted 4 March 2005

The Cdc14 dual-specificity phosphatases regulate key events in the eukaryotic cell cycle. However, little is known about the function of mammalian CDC14B family members. Here, we demonstrate that subcellular localization of CDC14B protein is cell cycle regulated. CDC14B can bind, bundle, and stabilize microtubules in vitro independently of its catalytic activity. Basic amino acid residues within the nucleolar targeting domain are important for both retaining CDC14B in the nucleolus and preventing microtubule bundling. Overexpression of CDC14B resulted in the formation of cytoplasmic CDC14B and microtubule bundles in interphase cells. These microtubule bundles were resistant to microtubule depolymerization reagents and enriched in acetylated {alpha}-tubulin. Expression of cytoplasmic forms of CDC14B impaired microtubule nucleation from the microtubule organization center. CDC14B is thus a novel microtubule-bundling and -stabilizing protein, whose regulated subcellular localization may help modulate spindle and microtubule dynamics in mitosis.

* Corresponding author. Mailing address: Life Sciences Division, Oak Ridge National Laboratory, Bethel Valley Rd., Oak Ridge, TN 37831. Phone: (865) 574-5396. Fax: (865) 574-5345. E-mail: ywa{at}ornl.gov.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, June 2005, p. 4541-4551, Vol. 25, No. 11
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.11.4541-4551.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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