This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Oevelen, C. J. C.
Right arrow Articles by Timmers, H. T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Oevelen, C. J. C.
Right arrow Articles by Timmers, H. T. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2005, p. 4863-4872, Vol. 25, No. 12
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.12.4863-4872.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Differential Requirement of SAGA Subunits for Mot1p and Taf1p Recruitment in Gene Activation{dagger}

Chris J. C. van Oevelen, Hetty A. A. M. van Teeffelen, and H. T. Marc Timmers*

Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Centre Utrecht, Utrecht, The Netherlands

Received 17 December 2004/ Returned for modification 24 January 2005/ Accepted 1 March 2005

Transcription activation in yeast (Saccharomyces cerevisiae) involves ordered recruitment of transcription factor complexes, such as TFIID, SAGA, and Mot1p. Previously, we showed that both Mot1p and Taf1p are recruited to the HXT2 and HXT4 genes, which encode hexose transporter proteins. Here, we show that SAGA also binds to the HXT2 and HXT4 promoters and plays a pivotal role in the recruitment of Mot1p and Taf1p. The deletion of either SPT3 or SPT8 reduces Mot1p binding to HXT2 and HXT4. Surprisingly, the deletion of GCN5 reduces Taf1p binding to both promoters. When GCN5 is deleted in spt3{Delta} or spt8{Delta} strains, neither Mot1p nor Taf1p binds, and this results in a diminished recruitment of TATA binding protein and polymerase II to the HXT4 but not the HXT2 promoter. This is reflected by the SAGA-dependent expression of HXT4. In contrast, SAGA-independent induction of HXT2 suggests a functional redundancy with other factors. A functional interplay of different SAGA subunits with Mot1p and Taf1p was supported by phenotypic analysis of MOT1 SAGA or TAF1/SAGA double mutant strains, which revealed novel genetic interactions between MOT1 and SPT8 and between TAF1 and GCN5. In conclusion, our data demonstrate functional links between SAGA, Mot1p, and TFIID in HXT gene regulation.

* Corresponding author. Mailing address: Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands. Phone: 31 30 253 8981. Fax: 31 30 253 9035. E-mail: h.t.m.timmers{at}med.uu.nl.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, June 2005, p. 4863-4872, Vol. 25, No. 12
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.12.4863-4872.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sprouse, R. O., Wells, M. N., Auble, D. T. (2009). TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo. J. Biol. Chem. 284: 4525-4535 [Abstract] [Full Text]  
  • Arnett, D. R., Jennings, J. L., Tabb, D. L., Link, A. J., Weil, P. A. (2008). A Proteomics Analysis of Yeast Mot1p Protein-Protein Associations: Insights into Mechanism. Mol. Cell. Proteomics 7: 2090-2106 [Abstract] [Full Text]  
  • van Werven, F. J., van Bakel, H., van Teeffelen, H. A.A.M., Altelaar, A.F. M., Koerkamp, M. G., Heck, A. J.R., Holstege, F. C.P., Timmers, H.Th. M. (2008). Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome. Genes Dev. 22: 2359-2369 [Abstract] [Full Text]  
  • Laprade, L., Rose, D., Winston, F. (2007). Characterization of New Spt3 and TATA-Binding Protein Mutants of Saccharomyces cerevisiae: Spt3 TBP Allele-Specific Interactions and Bypass of Spt8. Genetics 177: 2007-2017 [Abstract] [Full Text]  
  • James, N., Landrieux, E., Collart, M. A. (2007). A SAGA-Independent Function of SPT3 Mediates Transcriptional Deregulation in a Mutant of the Ccr4-Not Complex in Saccharomyces cerevisiae. Genetics 177: 123-135 [Abstract] [Full Text]  
  • van Werven, F. J., Timmers, H. Th. M. (2006). The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation. Nucleic Acids Res 34: e33-e33 [Abstract] [Full Text]  
  • van Oevelen, C. J. C., van Teeffelen, H. A. A. M., van Werven, F. J., Timmers, H. Th. M. (2006). Snf1p-dependent Spt-Ada-Gcn5-acetyltransferase (SAGA) Recruitment and Chromatin Remodeling Activities on the HXT2 and HXT4 Promoters. J. Biol. Chem. 281: 4523-4531 [Abstract] [Full Text]  
  • Biswas, D., Yu, Y., Mitra, D., Stillman, D. J. (2006). Genetic Interactions Between Nhp6 and Gcn5 With Mot1 and the Ccr4-Not Complex That Regulate Binding of TATA-Binding Protein in Saccharomyces cerevisiae. Genetics 172: 837-849 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»