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Molecular and Cellular Biology, June 2005, p. 4993-5010, Vol. 25, No. 12
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.12.4993-5010.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

CCT Chaperonin Complex Is Required for the Biogenesis of Functional Plk1{dagger}

Xiaoqi Liu,* Chin-Yo Lin,{ddagger} Ming Lei, Shi Yan, Tianhua Zhou, and Raymond L. Erikson

Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

Received 3 January 2005/ Returned for modification 1 February 2005/ Accepted 15 March 2005

Experiments from several different organisms have demonstrated that polo-like kinases are involved in many aspects of mitosis and cytokinesis. Here, we provide evidence to show that Plk1 associates with chaperonin-containing TCP1 complex (CCT) both in vitro and in vivo. Silencing of CCT by use of RNA interference (RNAi) in mammalian cells inhibits cell proliferation, decreases cell viability, causes cell cycle arrest with 4N DNA content, and leads to apoptosis. Depletion of CCT in well-synchronized HeLa cells causes cell cycle arrest at G2, as demonstrated by a low mitotic index and Cdc2 activity. Complete depletion of Plk1 in well-synchronized cells also leads to G2 block, suggesting that misfolded Plk1 might be responsible for the failure of CCT-depleted cells to enter mitosis. Moreover, partial depletion of CCT or Plk1 leads to mitotic arrest. Finally, the CCT-depleted cells reenter the cell cycle upon reintroduction of the purified constitutively active form of Plk1, indicating that Plk1 might be a CCT substrate.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 495-9686. Fax: (617) 495-0681. E-mail: liu13{at}fas.harvard.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Genome Institute of Singapore, Genome Building, #02-01, 60 Biopolis Street, Singapore 138672, Singapore.

Molecular and Cellular Biology, June 2005, p. 4993-5010, Vol. 25, No. 12
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.12.4993-5010.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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