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Molecular and Cellular Biology, June 2005, p. 5215-5225, Vol. 25, No. 12
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.12.5215-5225.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptional Regulation of the SCL Locus: Identification of an Enhancer That Targets the Primitive Erythroid Lineage In Vivo

E. Delabesse ,{dagger},{ddagger} S. Ogilvy,{ddagger} M. A. Chapman, S. G. Piltz, B. Gottgens, and A. R. Green*

University of Cambridge, Department of Hematology, Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, United Kingdom

Received 6 December 2004/ Returned for modification 16 January 2005/ Accepted 2 March 2005

The stem cell leukemia (SCL) gene, also known as TAL-1, encodes a basic helix-loop-helix protein that is essential for the formation of all hematopoietic lineages, including primitive erythropoiesis. Appropriate transcriptional regulation is essential for the biological functions of SCL, and we have previously identified five distinct enhancers which target different subdomains of the normal SCL expression pattern. However, it is not known whether these SCL enhancers also regulate neighboring genes within the SCL locus, and the erythroid expression of SCL remains unexplained. Here, we have quantitated transcripts from SCL and neighboring genes in multiple hematopoietic cell types. Our results show striking coexpression of SCL and its immediate downstream neighbor, MAP17, suggesting that they share regulatory elements. A systematic survey of histone H3 and H4 acetylation throughout the SCL locus in different hematopoietic cell types identified several peaks of histone acetylation between SIL and MAP17, all of which corresponded to previously characterized SCL enhancers or to the MAP17 promoter. Downstream of MAP17 (and 40 kb downstream of SCL exon 1a), an additional peak of acetylation was identified in hematopoietic cells and was found to correlate with expression of SCL but not other neighboring genes. This +40 region is conserved in human-dog-mouse-rat sequence comparisons, functions as an erythroid cell-restricted enhancer in vitro, and directs ß-galactosidase expression to primitive, but not definitive, erythroblasts in transgenic mice. The SCL +40 enhancer provides a powerful tool for studying the molecular and cellular biology of the primitive erythroid lineage.

* Corresponding author. Mailing address: University of Cambridge, Department of Hematology, CIMR, Hills Road, Cambridge CB2 2XY, United Kingdom. Phone: 44 1223 336 820. Fax: 44 1223 762 670. E-mail: arg1000{at}cam.ac.uk.

{dagger} Present address: Laboratory of Hematology and INSERM unit E02.10, Hôpital Necker-Enfants Malades, University Paris V, 149-161, rue de Sèvres, 75743 Paris cedex 15, France.

{ddagger} These authors contributed equally to this work.

Molecular and Cellular Biology, June 2005, p. 5215-5225, Vol. 25, No. 12
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.12.5215-5225.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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