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Molecular and Cellular Biology, July 2005, p. 5329-5338, Vol. 25, No. 13
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.13.5329-5338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of MuSK Expression by CREB Interacting with a CRE-Like Element and MyoD

Chang-Hoon Kim, Wen C. Xiong, and Lin Mei*

Program of Developmental Neurobiology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912

Received 23 November 2004/ Returned for modification 28 December 2004/ Accepted 8 April 2005

The type I receptor-like protein tyrosine kinase MuSK is essential for the neuromuscular junction formation. MuSK expression is tightly regulated during development, but the underlying mechanisms were unclear. Here we identified a novel mechanism by which MuSK expression may be regulated. A cyclic AMP response element (CRE)-like element in the 5'-flanking region of the MuSK gene binds to CREB1 (CRE-binding protein 1). Mutation of this element increases the MuSK promoter activity, suggesting a role for CREB1 in attenuation of MuSK expression. Interestingly, CREB mutants unable to bind to DNA also inhibit MuSK promoter activity, suggesting a CRE-independent inhibitory mechanism. In agreement, CREB1 could inhibit a mutant MuSK transgene reporter whose CRE site was mutated. We provide evidence that CREB interacts directly with MyoD, a myogenic factor essential for MuSK expression in muscle cells. Suppression of CREB expression by small interfering RNA increases MuSK promoter activity. These results demonstrate an important role for CREB1 in the regulation of MuSK expression.

* Corresponding author. Mailing address: Program of Developmental Neurobiology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, CB2803, 1120 15th Street, Augusta, GA 30912. Phone: (706) 721-8775. Fax: (706) 721-8685. E-mail: lmei{at}mcg.edu.

Molecular and Cellular Biology, July 2005, p. 5329-5338, Vol. 25, No. 13
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.13.5329-5338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Cottle, D. L., McGrath, M. J., Cowling, B. S., Coghill, I. D., Brown, S., Mitchell, C. A. (2007). FHL3 binds MyoD and negatively regulates myotube formation. J. Cell Sci. 120: 1423-1435 [Abstract] [Full Text]  
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