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Molecular and Cellular Biology, July 2005, p. 5590-5598, Vol. 25, No. 13
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.13.5590-5598.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

DREF Is Required for Efficient Growth and Cell Cycle Progression in Drosophila Imaginal Discs

Joogyung Hyun, Heinrich Jasper, and Dirk Bohmann*

Department of Biomedical Genetics, The Aab Institute of Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 633, Rochester, NY 14642

Received 15 December 2004/ Returned for modification 26 January 2005/ Accepted 4 April 2005

Based on overexpression studies and target gene analyses, the transcription factor DNA replication-related element factor (DREF) has been proposed to regulate growth and replication in Drosophila melanogaster. Here we present loss-of-function experiments to analyze the contribution of DREF to these processes. RNA interference-mediated extinction of DREF function in vivo demonstrates a requirement for the protein for normal progression through the cell cycle and consequently for growth of imaginal discs and the derived adult organs. We show that DREF regulates the expression of genes that are required for the transition of imaginal disc cells through S phase. In conditions of suppressed apoptosis, DREF activation can cause overgrowth of developing organs. These data establish DREF as a global regulator of transcriptional programs that mediate cell proliferation and organ growth during animal development.

* Corresponding author. Mailing address: Department of Biomedical Genetics, University of Rochester Medical Center, 601 Elmwood Avenue, Box 633, Rochester, NY 14642. Phone: (585) 273-1446. Fax: (585) 273-1450. E-mail: dirk_bohmann{at}urmc.rochester.edu.

Molecular and Cellular Biology, July 2005, p. 5590-5598, Vol. 25, No. 13
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.13.5590-5598.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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