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Molecular and Cellular Biology, July 2005, p. 6267-6278, Vol. 25, No. 14
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.14.6267-6278.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cardiac Tissue-Specific Repression of CELF Activity Disrupts Alternative Splicing and Causes Cardiomyopathy{dagger}

Andrea N. Ladd,1,{ddagger} George Taffet,3 Craig Hartley,3 Debra L. Kearney,1 and Thomas A. Cooper1,2*

Departments of Pathology,1 Molecular and Cellular Biology,2 Medicine, Baylor College of Medicine, Houston, Texas 770303

Received 18 March 2005/ Returned for modification 12 April 2005/ Accepted 19 April 2005

Members of the CELF family of RNA binding proteins have been implicated in alternative splicing regulation in developing heart. Transgenic mice that express a nuclear dominant-negative CELF protein specifically in the heart (MHC-CELF{Delta}) develop cardiac hypertrophy and dilated cardiomyopathy with defects in alternative splicing beginning as early as 3 weeks after birth. MHC-CELF{Delta} mice exhibit extensive cardiac fibrosis, severe cardiac dysfunction, and premature death. Interestingly, the penetrance of the phenotype is greater in females than in males despite similar levels of dominant-negative expression, suggesting that there is sex-specific modulation of splicing activity. The cardiac defects in MHC-CELF{Delta} mice are directly attributable to reduced levels of CELF activity, as crossing these mice with mice overexpressing CUG-BP1, a wild-type CELF protein, rescues defects in alternative splicing, the severity and incidence of cardiac hypertrophy, and survival. We conclude that CELF protein activity is required for normal alternative splicing in the heart in vivo and that normal CELF-mediated alternative splicing regulation is in turn required for normal cardiac function.

* Corresponding author. Mailing address: One Baylor Plaza, Rm. 268B, Houston, TX 77030. Phone: (713) 798-3141. Fax: (713) 798-5838. E-mail: tcooper{at}bcm.tmc.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} Present address: Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195.

Molecular and Cellular Biology, July 2005, p. 6267-6278, Vol. 25, No. 14
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.14.6267-6278.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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