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Molecular and Cellular Biology, August 2005, p. 6789-6797, Vol. 25, No. 15
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.15.6789-6797.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

DNA Replication Origin Plasticity and Perturbed Fork Progression in Human Inverted Repeats

Ronald Lebofsky and Aaron Bensimon*

Institut Pasteur, Unité de Stabilité des Génomes, Département Structure et Dynamique des Génomes, 25 rue du Dr. Roux, 75724 Paris cedex 15, France

Received 9 February 2005/ Returned for modification 15 March 2005/ Accepted 1 May 2005

The stability of metazoan genomes during their duplication depends on the spatiotemporal activation of origins and the progression of forks. Human rRNA genes represent a unique challenge to DNA replication since a large proportion of them exist as noncanonical palindromes in addition to canonical tandem repeats. Whether origin usage and/or fork elongation can cope with the variable structure of these genes is unknown. By analyzing single combed DNA molecules from HeLa cells, we studied the rRNA gene replication program according to the organization of canonical versus noncanonical rRNA genes. Origin positioning, spacing, and timing were not affected by the underlying rRNA gene physical structure. Conversely, fork arrest, both temporary and permanent, occurred more frequently when rRNA gene palindromes were encountered. These findings reveal that while initiation mechanisms are flexible enough to adapt to an rRNA gene structure of any arrangement, palindromes represent obstacles to fork progression, which is a likely source of genomic instability.

* Corresponding author. Mailing address: Institut Pasteur, Unité de Stabilité des Génomes, Département Structure et Dynamique des Génomes, 25 rue du Dr. Roux, 75724 Paris cedex 15, France. Phone: 33 (0)1 40 61 32 40. Fax: 33 (0)1 45 68 87 90. E-mail: abensim{at}pasteur.fr.

Molecular and Cellular Biology, August 2005, p. 6789-6797, Vol. 25, No. 15
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.15.6789-6797.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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