This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bader, B. L.
Right arrow Articles by Nischt, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bader, B. L.
Right arrow Articles by Nischt, R.

 Previous Article

Molecular and Cellular Biology, August 2005, p. 6846-6856, Vol. 25, No. 15
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.15.6846-6856.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Compound Genetic Ablation of Nidogen 1 and 2 Causes Basement Membrane Defects and Perinatal Lethality in Mice

Bernhard L. Bader ,1,{dagger},{ddagger} Neil Smyth,2,{dagger},§ Sabine Nedbal,1 Nicolai Miosge,3 Anke Baranowsky,4 Sharada Mokkapati,4 Monzur Murshed,5 and Roswitha Nischt4*

Department of Molecular Biology, Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany,1 Institute for Biochemistry II and Center of Molecular Medicine, University of Cologne, D-50924 Cologne, Germany,2 Department of Histology, University of Göttingen, D-37075 Göttingen, Germany,3 Department of Dermatology, University of Cologne, D-50924 Cologne, Germany,4 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 770305

Received 21 December 2004/ Returned for modification 24 February 2005/ Accepted 4 May 2005

Nidogen 1 and 2 are basement membrane glycoproteins, and previous biochemical and functional studies indicate that they may play a crucial role in basement membrane assembly. While they show a divergent expression pattern in certain adult tissues, both have a similar distribution during development. Gene knockout studies in mice demonstrated that the loss of either isoform has no effect on basement membrane formation and organ development, suggesting complementary functions. Here, we show that this is indeed the case. Deficiency of both nidogens in mice resulted in perinatal lethality. Nidogen 1 and 2 do not appear to be crucial in establishing tissue architecture during organ development; instead, they are essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue. These organ defects are not compatible with postnatal survival. Ultrastructural analysis suggests that the phenotypes directly result from basement membrane changes. However, despite the ubiquitous presence of nidogens in basement membranes, defects do not occur in all tissues or in all basement membranes, suggesting a varying spectrum of roles for nidogens in the basement membrane.

* Corresponding author. Mailing address: Department of Dermatology, University of Cologne, 50924 Cologne, Germany. Phone: 49-221-478-5472. Fax: 49-221-478-86133. E-mail: Roswitha.Nischt{at}uni-koeln.de.

{dagger} B.L.B. and N.S. contributed equally to this work.

{ddagger} Present address: Department of Nutritional Medicine, Technical University Munich, D-85350 Freising, Germany.

§ Present address: School of Biological Sciences, University of Southampton, Southampton, United Kingdom.

Molecular and Cellular Biology, August 2005, p. 6846-6856, Vol. 25, No. 15
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.15.6846-6856.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McKee, K. K., Capizzi, S., Yurchenco, P. D. (2009). Scaffold-forming and Adhesive Contributions of Synthetic Laminin-binding Proteins to Basement Membrane Assembly. J. Biol. Chem. 284: 8984-8994 [Abstract] [Full Text]  
  • Malan, D., Reppel, M., Dobrowolski, R., Roell, W., Smyth, N., Hescheler, J., Paulsson, M., Bloch, W., Fleischmann, B. K. (2009). Lack of Laminin {gamma}1 in Embryonic Stem Cell-Derived Cardiomyocytes Causes Inhomogeneous Electrical Spreading Despite Intact Differentiation and Function. Stem Cells 27: 88-99 [Abstract] [Full Text]  
  • Kubota, Y., Ohkura, K., Tamai, K. K., Nagata, K., Nishiwaki, K. (2008). MIG-17/ADAMTS controls cell migration by recruiting nidogen to the basement membrane in C. elegans. Proc. Natl. Acad. Sci. USA 105: 20804-20809 [Abstract] [Full Text]  
  • Arrell, D. K., Niederlander, N. J., Faustino, R. S., Behfar, A., Terzic, A. (2008). Cardioinductive Network Guiding Stem Cell Differentiation Revealed by Proteomic Cartography of Tumor Necrosis Factor {alpha}-Primed Endodermal Secretome. Stem Cells 26: 387-400 [Abstract] [Full Text]  
  • Fujiwara, H., Hayashi, Y., Sanzen, N., Kobayashi, R., Weber, C. N., Emoto, T., Futaki, S., Niwa, H., Murray, P., Edgar, D., Sekiguchi, K. (2007). Regulation of Mesodermal Differentiation of Mouse Embryonic Stem Cells by Basement Membranes. J. Biol. Chem. 282: 29701-29711 [Abstract] [Full Text]  
  • LeBleu, V. S., MacDonald, B., Kalluri, R. (2007). Structure and Function of Basement Membranes. Exp. Biol. Med. 232: 1121-1129 [Abstract] [Full Text]  
  • McKee, K. K., Harrison, D., Capizzi, S., Yurchenco, P. D. (2007). Role of Laminin Terminal Globular Domains in Basement Membrane Assembly. J. Biol. Chem. 282: 21437-21447 [Abstract] [Full Text]  
  • Bose, K., Nischt, R., Page, A., Bader, B. L., Paulsson, M., Smyth, N. (2006). Loss of Nidogen-1 and -2 Results in Syndactyly and Changes in Limb Development. J. Biol. Chem. 281: 39620-39629 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»