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Molecular and Cellular Biology, August 2005, p. 6879-6888, Vol. 25, No. 16
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.16.6879-6888.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Translation Termination Is Involved in Histone mRNA Degradation when DNA Replication Is Inhibited

Handan Kaygun1,2 and William F. Marzluff1,2,3*

Department of Biology,1 Program in Molecular Biology and Biotechnology,2 Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 275993

Received 18 February 2005/ Returned for modification 23 March 2005/ Accepted 31 May 2005

The levels of replication-dependent histone mRNAs are coordinately regulated with DNA synthesis. A major regulatory step in histone mRNA metabolism is regulation of the half-life of histone mRNAs. Replication-dependent histone mRNAs are the only metazoan mRNAs that are not polyadenylated. Instead, they end with a conserved stem-loop structure, which is recognized by the stem-loop binding protein (SLBP). SLBP is required for histone mRNA processing, as well as translation. We show here, using histone mRNAs whose translation can be regulated by the iron response element, that histone mRNAs need to be actively translated for their rapid degradation following the inhibition of DNA synthesis. We also demonstrate the requirement for translation using a mutant SLBP which is inactive in translation. Histone mRNAs are not rapidly degraded when DNA synthesis is inhibited or at the end of S phase in cells expressing this mutant SLBP. Replication-dependent histone mRNAs have very short 3' untranslated regions, with the stem-loop located 30 to 70 nucleotides downstream of the translation termination codon. We show here that the stability of histone mRNAs can be modified by altering the position of the stem-loop, thereby changing the distance from the translation termination codon.

* Corresponding author. Mailing address: Program in Molecular Biology and Biotechnology, CB #7100, University of North Carolina, Chapel Hill, NC 27599. Phone: (919) 962-8920. Fax: (919) 966-6821. E-mail: marzluff{at}med.unc.edu.

Molecular and Cellular Biology, August 2005, p. 6879-6888, Vol. 25, No. 16
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.16.6879-6888.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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