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Molecular and Cellular Biology, September 2005, p. 7459-7472, Vol. 25, No. 17
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.17.7459-7472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Severe Adenine Starvation Activates Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae

Anne-Laure Todeschini, Antonin Morillon, Mathias Springer, and Pascale Lesage*

Institut de Biologie Physico-Chimique, CNRS-UPR 9073, 13 rue Pierre & Marie Curie, F-75005 Paris, France

Received 23 February 2005/ Returned for modification 11 April 2005/ Accepted 2 June 2005

Ty1 retrotransposons of the yeast Saccharomyces cerevisiae are activated by different kinds of stress. Here we show that Ty1 transcription is stimulated under severe adenine starvation conditions. The Bas1 transcriptional activator, responsible for the induction of genes of the de novo AMP biosynthesis pathway (ADE) in the absence of adenine, is not involved in this response. Activation occurs mainly on Ty1 elements, whose expression is normally repressed by chromatin and is suppressed in a hta1-htb1{Delta} mutant that alters chromatin structure. Activation is also abolished in a snf2{Delta} mutant. Several regions of the Ty1 promoter are necessary to achieve full activation, suggesting that full integrity of the promoter sequences might be important for activation. Together, these observations are consistent with a model in which the activation mechanism involves chromatin remodeling at Ty1 promoters. The consequence of Ty1 transcriptional activation in response to adenine starvation is an increase in Ty1 cDNA levels and a relief of Ty1 dormancy. The retrotransposition of four native Ty1 elements increases in proportion to their increase in transcription. Implications for the regulation of Ty1 mobility by changes in Ty1 mRNA levels are discussed.

* Corresponding author. Mailing address: IBPC, CNRS UPR 9073, 13 rue Pierre et Marie Curie, F-75005 Paris, France. Phone: (33) 1 58 41 51 25. Fax: (33) 1 58 41 50 20. E-mail: lesage{at}ibpc.fr.

Molecular and Cellular Biology, September 2005, p. 7459-7472, Vol. 25, No. 17
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.17.7459-7472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Servant, G., Pennetier, C., Lesage, P. (2008). Remodeling Yeast Gene Transcription by Activating the Ty1 Long Terminal Repeat Retrotransposon under Severe Adenine Deficiency. Mol. Cell. Biol. 28: 5543-5554 [Abstract] [Full Text]  
  • Nyswaner, K. M., Checkley, M. A., Yi, M., Stephens, R. M., Garfinkel, D. J. (2008). Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis. Genetics 178: 197-214 [Abstract] [Full Text]  
  • Wilhelm, M., Wilhelm, F.-X. (2006). Cooperation between Reverse Transcriptase and Integrase during Reverse Transcription and Formation of the Preintegrative Complex of Ty1.. Eukaryot Cell 5: 1760-1769 [Abstract] [Full Text]  


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