This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morishita, Y.
Right arrow Articles by Ishibashi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morishita, Y.
Right arrow Articles by Ishibashi, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2005, p. 7770-7779, Vol. 25, No. 17
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.17.7770-7779.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Disruption of Aquaporin-11 Produces Polycystic Kidneys following Vacuolization of the Proximal Tubule

Yoshiyuki Morishita,1 Toshiyuki Matsuzaki,2 Mariko Hara-chikuma,3 Ayaka Andoo,4 Mariko Shimono,4 Asako Matsuki,5 Katsuki Kobayashi,7 Masahiro Ikeda,4 Tadashi Yamamoto,5 Alan Verkman,3 Eiji Kusano,1 Shigeo Ookawara,1 Kuniaki Takata,2 Sei Sasaki,6 and Kenichi Ishibashi1,6,7*

Department of Nephrology and Anatomy, Jichi Medical School, Tochigi 329-0498, Japan,1 Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan,2 Cardiovascular Research Institute, UCSF, San Francisco, California 94143-0521,3 Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan,4 Institute of Nephrology, Niigata University Graduate School of Medical and Dental Science, Niigata 951-8510, Japan,5 Department of Nephrology, Tokyo Medical and Dental University, Tokyo 113-8591, Japan,6 Clinical Research Center, Chiba-East National Hospital, Chiba 260-8712, Japan7

Received 18 March 2005/ Returned for modification 6 May 2005/ Accepted 9 June 2005

Aquaporin-11 (AQP11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes, but its function is unknown. We investigated its potential contribution to the kidney. Immunohistochemistry revealed that AQP11 was localized intracellularly in the proximal tubule. When AQP11 was transfected in CHO-K1 cells, it was localized in intracellular organelles. AQP11-null mice were generated; these mice exhibited vacuolization and cyst formation of the proximal tubule. AQP11-null mice were born normally but died before weaning due to advanced renal failure with polycystic kidneys, in which cysts occupied the whole cortex. Remarkably, cyst epithelia contained vacuoles. These vacuoles were present in the proximal tubules of newborn mice. In 3-week-old mice, these tubules contained multiple cysts. Primary cultured cells of the proximal tubule revealed an endosomal acidification defect in AQP11-null mice. These data demonstrate that AQP11 is essential for the proximal tubular function. AQP11-null mice are a novel model for polycystic kidney diseases and will provide a new mechanism for cystogenesis.

* Corresponding author. Mailing address: Department of Molecular Biology Research, Institute of Clinical Investigation, Chiba-East National Hospital, National Hospital Organization, 673 Nitona, Chuou-ku, Chiba 260-8712, Japan. Phone: 81-43-261-5171. Fax: 81-43-268-2613. E-mail: kishiba{at}nitona.hosp.go.jp.

Molecular and Cellular Biology, September 2005, p. 7770-7779, Vol. 25, No. 17
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.17.7770-7779.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hermo, L., Schellenberg, M., Liu, L. Y., Dayanandan, B., Zhang, T., Mandato, C. A., Smith, C. E. (2008). Membrane Domain Specificity in the Spatial Distribution of Aquaporins 5, 7, 9, and 11 in Efferent Ducts and Epididymis of Rats. J. Histochem. Cytochem. 56: 1121-1135 [Abstract] [Full Text]  
  • Calamita, G., Ferri, D., Gena, P., Carreras, F. I., Liquori, G. E., Portincasa, P., Marinelli, R. A., Svelto, M. (2008). Altered expression and distribution of aquaporin-9 in the liver of rat with obstructive extrahepatic cholestasis. Am. J. Physiol. Gastrointest. Liver Physiol. 295: G682-G690 [Abstract] [Full Text]  
  • Okada, S., Misaka, T., Tanaka, Y., Matsumoto, I., Ishibashi, K., Sasaki, S., Abe, K. (2008). Aquaporin-11 knockout mice and polycystic kidney disease animals share a common mechanism of cyst formation. FASEB J. 22: 3672-3684 [Abstract] [Full Text]  
  • Ablimit, A., Aoki, T., Matsuzaki, T., Suzuki, T., Hagiwara, H., Takami, S., Takata, K. (2008). Immunolocalization of Water Channel Aquaporins in the Vomeronasal Organ of the Rat: Expression of AQP4 in Neuronal Sensory Cells. Chem Senses 33: 481-488 [Abstract] [Full Text]  
  • Weber, K. H., Lee, E. K., Basavanna, U., Lindley, S., Ziegelstein, R. C., Germino, G. G., Sutters, M. (2008). Heterologous expression of polycystin-1 inhibits endoplasmic reticulum calcium leak in stably transfected MDCK cells. Am. J. Physiol. Renal Physiol. 294: F1279-F1286 [Abstract] [Full Text]  
  • Burtey, S., Riera, M., Ribe, E., Pennekamp, P., Passage, E., Rance, R., Dworniczak, B., Fontes, M. (2008). Overexpression of PKD2 in the mouse is associated with renal tubulopathy. Nephrol Dial Transplant 23: 1157-1165 [Abstract] [Full Text]  
  • Butler, T. L., Au, C. G., Yang, B., Egan, J. R., Tan, Y. M., Hardeman, E. C., North, K. N., Verkman, A. S., Winlaw, D. S. (2006). Cardiac aquaporin expression in humans, rats, and mice. Am. J. Physiol. Heart Circ. Physiol. 291: H705-H713 [Abstract] [Full Text]  
  • Matsuzaki, T., Ablimit, A., Suzuki, T., Aoki, T., Hagiwara, H., Takata, K. (2006). Changes of aquaporin 5-distribution during release and reaccumulation of secretory granules in isoproterenol-treated mouse parotid gland. J Electron Microsc (Tokyo) 55: 183-189 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»