This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodrik, V.
Right arrow Articles by Foster, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodrik, V.
Right arrow Articles by Foster, D. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2005, p. 7917-7925, Vol. 25, No. 17
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.17.7917-7925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Survival Signals Generated by Estrogen and Phospholipase D in MCF-7 Breast Cancer Cells Are Dependent on Myc

Vanessa Rodrik, Yang Zheng, Faith Harrow, Yuhong Chen, and David A. Foster*

Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, New York 10021

Received 9 December 2004/ Returned for modification 17 February 2005/ Accepted 3 June 2005

Estrogens, which have been strongly implicated in the development of breast cancer, enhance proliferation of mammary epithelial cells and, importantly, estrogen receptor (ER)-positive breast cancer cells. In the absence of serum growth factors, the ER-positive MCF-7 breast cancer cell line undergoes apoptosis. Estrogens, most commonly 17-ß-estradiol (E2), can suppress apoptosis in MCF-7 cells deprived of serum. While E2 stimulated a short-term transient increase in Myc expression, E2 stimulated a sustained increase in Myc expression that was detectable at 48 h and pronounced at 5 days, the point where increased proliferation of MCF-7 cells in the absence of serum could be detected. The delayed increase in Myc expression was not dependent upon transcription of the Myc gene. Suppression of Myc expression reversed the survival effects of E2. The Myc-dependent survival signal generated by E2 was dependent upon basal levels of mTOR (mammalian target of rapamycin) and two upstream regulators of mTOR, phosphatidylinositol 3-kinase and phospholipase D (PLD). Stable elevated expression of PLD2 also increased Myc expression and provided a Myc-dependent survival signal in the absence of E2. These data provide evidence that E2 promotes survival signals in breast cancer cells through an mTOR-dependent increase in Myc expression. The data also suggest that elevated PLD expression, which is common in breast cancer, confers E2 independence.

* Corresponding author. Mailing address: Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Avenue, New York, NY 10021. Phone: (212) 772-4075. Fax: (212) 772-5227. E-mail: foster{at}genectr.hunter.cuny.edu.

Molecular and Cellular Biology, September 2005, p. 7917-7925, Vol. 25, No. 17
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.17.7917-7925.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pedram, A., Razandi, M., Evinger, A. J., Lee, E., Levin, E. R. (2009). Estrogen Inhibits ATR Signaling to Cell Cycle Checkpoints and DNA Repair. Mol. Biol. Cell 20: 3374-3389 [Abstract] [Full Text]  
  • Knoepp, S. M., Chahal, M. S., Xie, Y., Zhang, Z., Brauner, D. J., Hallman, M. A., Robinson, S. A., Han, S., Imai, M., Tomlinson, S., Meier, K. E. (2008). Effects of Active and Inactive Phospholipase D2 on Signal Transduction, Adhesion, Migration, Invasion, and Metastasis in EL4 Lymphoma Cells. Mol. Pharmacol. 74: 574-584 [Abstract] [Full Text]  
  • Shi, B., Liang, J., Yang, X., Wang, Y., Zhao, Y., Wu, H., Sun, L., Zhang, Y., Chen, Y., Li, R., Zhang, Y., Hong, M., Shang, Y. (2007). Integration of Estrogen and Wnt Signaling Circuits by the Polycomb Group Protein EZH2 in Breast Cancer Cells. Mol. Cell. Biol. 27: 5105-5119 [Abstract] [Full Text]  
  • Yu, J., Henske, E. P. (2006). Estrogen-Induced Activation of Mammalian Target of Rapamycin Is Mediated via Tuberin and the Small GTPase Ras Homologue Enriched in Brain. Cancer Res. 66: 9461-9466 [Abstract] [Full Text]  
  • Li, L., Cheng, A. S. L., Jin, V. X., Paik, H. H., Fan, M., Li, X., Zhang, W., Robarge, J., Balch, C., Davuluri, R. V., Kim, S., Huang, T. H.-M., Nephew, K. P. (2006). A mixture model-based discriminate analysis for identifying ordered transcription factor binding site pairs in gene promoters directly regulated by estrogen receptor-{alpha}. Bioinformatics 22: 2210-2216 [Abstract] [Full Text]  
  • Zheng, Y., Rodrik, V., Toschi, A., Shi, M., Hui, L., Shen, Y., Foster, D. A. (2006). Phospholipase D Couples Survival and Migration Signals in Stress Response of Human Cancer Cells. J. Biol. Chem. 281: 15862-15868 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»