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Molecular and Cellular Biology, September 2005, p. 8166-8178, Vol. 25, No. 18
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.18.8166-8178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Pocket Protein Complexes Are Recruited to Distinct Targets in Quiescent and Proliferating Cells

Egle Balciunaite,1 Alexander Spektor,1 Nathan H. Lents,1 Hugh Cam,1 Hein te Riele,2 Anthony Scime,3 Michael A. Rudnicki,3 Richard Young,4 and Brian David Dynlacht1*

Department of Pathology, New York University School of Medicine and New York University Cancer Institute, New York, New York,1 Division of Molecular Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands,2 Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1H 8L6,3 Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 021424

Received 4 April 2005/ Returned for modification 26 May 2005/ Accepted 27 June 2005

Biochemical and genetic studies have determined that retinoblastoma protein (pRB) tumor suppressor family members have overlapping functions. However, these studies have largely failed to distinguish functional differences between the highly related p107 and p130 proteins. Moreover, most studies pertaining to the pRB family and its principal target, the E2F transcription factor, have focused on cells that have reinitiated a cell cycle from quiescence, although recent studies suggest that cycling cells exhibit layers of regulation distinct from mitogenically stimulated cells. Using genome-wide chromatin immunoprecipitation, we show that there are distinct classes of genes directly regulated by unique combinations of E2F4, p107, and p130, including a group of genes specifically regulated in cycling cells. These groups exhibit both distinct histone acetylation signatures and patterns of mammalian Sin3B corepressor recruitment. Our findings suggest that cell cycle-dependent repression results from recruitment of an unexpected array of diverse complexes and reveals specific differences between transcriptional regulation in cycling and quiescent cells. In addition, factor location analyses have, for the first time, allowed the identification of novel and specific targets of the highly related transcriptional regulators p107 and p130, suggesting new and distinct regulatory networks engaged by each protein in continuously cycling cells.

* Corresponding author. Mailing address: Department of Pathology, MSB 504, New York University School of Medicine and New York University Cancer Institute, 550 First Avenue, New York, NY 10016. Phone: 212-263-6162. Fax: 212-263-6162. E-mail: brian.dynlacht{at}med.nyu.edu.

Molecular and Cellular Biology, September 2005, p. 8166-8178, Vol. 25, No. 18
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.18.8166-8178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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