This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Watrin, E.
Right arrow Articles by Legagneux, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watrin, E.
Right arrow Articles by Legagneux, V.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2005, p. 740-750, Vol. 25, No. 2
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.2.740-750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Contribution of hCAP-D2, a Non-SMC Subunit of Condensin I, to Chromosome and Chromosomal Protein Dynamics during Mitosis

Erwan Watrin* and Vincent Legagneux

UMR 6061 CNRS, Université de Rennes 1, Faculté de Médecine, Rennes, France

Received 13 July 2004/ Returned for modification 11 August 2004/ Accepted 19 October 2004

Condensins are heteropentameric complexes that were first identified as structural components of mitotic chromosomes. They are composed of two SMC (structural maintenance of chromosomes) and three non-SMC subunits. Condensins play a role in the resolution and segregation of sister chromatids during mitosis, as well as in some aspects of mitotic chromosome assembly. Two distinct condensin complexes, condensin I and condensin II, which differ only in their non-SMC subunits, exist. Here, we used an RNA interference approach to deplete hCAP-D2, a non-SMC subunit of condensin I, in HeLa cells. We found that the association of hCAP-H, another non-SMC subunit of condensin I, with mitotic chromosomes depends on the presence of hCAP-D2. Moreover, chromatid axes, as defined by topoisomerase II and hCAP-E localization, are disorganized in the absence of hCAP-D2, and the resolution and segregation of sister chromatids are impaired. In addition, hCAP-D2 depletion affects chromosome alignment in metaphase and delays entry into anaphase. This suggests that condensin I is involved in the correct attachment between chromosome kinetochores and microtubules of the mitotic spindle. These results are discussed relative to the effects of depleting both condensin complexes.

* Corresponding author. Present address: Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, 1030 Vienna, Austria. Phone: 00 43 1 797 30 625. Fax: 00 43 1 798 71 53. E-mail: watrin{at}imp.univie.ac.at.

Molecular and Cellular Biology, January 2005, p. 740-750, Vol. 25, No. 2
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.2.740-750.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Uchida, K. S.K., Takagaki, K., Kumada, K., Hirayama, Y., Noda, T., Hirota, T. (2009). Kinetochore stretching inactivates the spindle assembly checkpoint. JCB 184: 383-390 [Abstract] [Full Text]  
  • Roca, J. (2009). Topoisomerase II: a fitted mechanism for the chromatin landscape. Nucleic Acids Res 37: 721-730 [Abstract] [Full Text]  
  • Scibetta, A. G., Santangelo, S., Coleman, J., Hall, D., Chaplin, T., Copier, J., Catchpole, S., Burchell, J., Taylor-Papadimitriou, J. (2007). Functional Analysis of the Transcription Repressor PLU-1/JARID1B. Mol. Cell. Biol. 27: 7220-7235 [Abstract] [Full Text]  
  • Gosling, K. M., Makaroff, L. E., Theodoratos, A., Kim, Y.-H., Whittle, B., Rui, L., Wu, H., Hong, N. A., Kennedy, G. C., Fritz, J.-A., Yates, A. L., Goodnow, C. C., Fahrer, A. M. (2007). A mutation in a chromosome condensin II subunit, kleisin beta, specifically disrupts T cell development. Proc. Natl. Acad. Sci. USA 104: 12445-12450 [Abstract] [Full Text]  
  • Blank, M., Lerenthal, Y., Mittelman, L., Shiloh, Y. (2006). Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage. JCB 174: 195-206 [Abstract] [Full Text]  
  • Oliveira, R. A., Coelho, P. A., Sunkel, C. E. (2005). The Condensin I Subunit Barren/CAP-H Is Essential for the Structural Integrity of Centromeric Heterochromatin during Mitosis. Mol. Cell. Biol. 25: 8971-8984 [Abstract] [Full Text]  
  • Losada, A., Hirano, T. (2005). Dynamic molecular linkers of the genome: the first decade of SMC proteins. Genes Dev. 19: 1269-1287 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»