This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rath, N. Articles by Morrisey, E. E. Search for Related Content PubMed PubMed Citation Articles by Rath, N. Articles by Morrisey, E. E.

LMCD1/Dyxin Is a Novel Transcriptional Cofactor That Restricts GATA6 Function by Inhibiting DNA Binding

Department of Medicine and Molecular Cardiology Research Center,¹ Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104²

Received 19 November 2004/ Returned for modification 30 December 2004/ Accepted 16 July 2005

The activity of GATA factors is regulated, in part, at the level of protein-protein interactions. LIM domain proteins, first defined by the zinc finger motifs found in the Lin11, Isl-1, and Mec-3 proteins, act as coactivators of GATA function in both hematopoietic and cardiovascular tissues. We have identified a novel GATA-LIM interaction between GATA6 and LMCD1/dyxin. The LIM domains and cysteine-rich domains in LMCD1/dyxin and the carboxy-terminal zinc finger of GATA6 mediate this interaction. Expression of LMCD1/dyxin is remarkably similar to that of GATA6, with high-level expression observed in distal airway epithelium of the lung, vascular smooth muscle, and myocardium. In contrast to other GATA-LIM protein interactions, LMCD1/dyxin represses GATA6 activation of both lung and cardiac tissue-specific promoters. Electrophoretic mobility shift and chromatin immunoprecipitation assays show that LMCD1/dyxin represses GATA6 function by inhibiting GATA6 DNA binding. These data reveal an interaction between GATA6 and LMCD1/dyxin and demonstrate a novel mechanism through which LIM proteins can assert their role as transcriptional cofactors of GATA proteins.

This article has been cited by other articles:

• Avivi, A., Brodsky, L., Nevo, E., Band, M. R. (2007). Differential expression profiling of the blind subterranean mole rat Spalax ehrenbergi superspecies: bioprospecting for hypoxia tolerance. Physiol. Genomics 27: 54-64 [Abstract] [Full Text]  
• Herring, B. P., El-Mounayri, O., Gallagher, P. J., Yin, F., Zhou, J. (2006). Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues. Am. J. Physiol. Cell Physiol. 291: C817-C827 [Abstract] [Full Text]