This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, Y.-N.
Right arrow Articles by Razin, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, Y.-N.
Right arrow Articles by Razin, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2005, p. 8904-8912, Vol. 25, No. 20
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.20.8904-8912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nonconventional Involvement of LysRS in the Molecular Mechanism of USF2 Transcriptional Activity in Fc{varepsilon}RI-Activated Mast Cells

Yu-Nee Lee and Ehud Razin*

Department of Biochemistry, Hebrew University Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel

Received 24 February 2005/ Returned for modification 7 April 2005/ Accepted 18 July 2005

Reports of the biological multifunctional activity of various aminoacyl tRNA synthetases have recently accumulated in the literature. The primary function of these critical enzymes is to charge various tRNAs with their appropriate amino acids, thus producing the building blocks of protein synthesis. We have previously shown that lysyl tRNA synthetase (LysRS) associates with microphthalmia transcription factor (MITF) and regulates its activity by synthesis of Ap4A in mast cells. Here, we show for the first time that LysRS associates with another transcription factor, USF2, which unlike MITF, is ubiquitously expressed in eukaryotic cells. Using mast cells, we have found that USF2 is negatively regulated by Hint and Ap4A acts as a positive regulator of USF2 by a molecular mechanism similar to that described for MITF. Since USF2 plays a significant role in a variety of cellular functions, our finding suggests that LysRS and Ap4A may be involved in general regulation of gene transcription.

* Corresponding author. Mailing address: Department of Biochemistry, Hebrew University Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel. Phone: 972 2 675 8288. Fax: 972 2 675 7379. E-mail: ehudr{at}cc.huji.ac.il.

Molecular and Cellular Biology, October 2005, p. 8904-8912, Vol. 25, No. 20
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.20.8904-8912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fukui, H., Hanaoka, R., Kawahara, A. (2009). Noncanonical Activity of Seryl-tRNA Synthetase Is Involved in Vascular Development. Circ. Res. 104: 1253-1259 [Abstract] [Full Text]  
  • Kaminska, M., Havrylenko, S., Decottignies, P., Le Marechal, P., Negrutskii, B., Mirande, M. (2009). Dynamic Organization of Aminoacyl-tRNA Synthetase Complexes in the Cytoplasm of Human Cells. J. Biol. Chem. 284: 13746-13754 [Abstract] [Full Text]  
  • Cen, B., Li, H., Weinstein, I. B. (2009). Histidine Triad Nucleotide-binding Protein 1 Up-regulates Cellular Levels of p27KIP1 by Targeting ScfSKP2 Ubiquitin Ligase and Src. J. Biol. Chem. 284: 5265-5276 [Abstract] [Full Text]  
  • Carmi-Levy, I., Yannay-Cohen, N., Kay, G., Razin, E., Nechushtan, H. (2008). Diadenosine Tetraphosphate Hydrolase Is Part of the Transcriptional Regulation Network in Immunologically Activated Mast Cells. Mol. Cell. Biol. 28: 5777-5784 [Abstract] [Full Text]  
  • Chou, T.-F., Tikh, I. B., Horta, B. A. C., Ghosh, B., De Alencastro, R. B., Wagner, C. R. (2007). Engineered Monomeric Human Histidine Triad Nucleotide-binding Protein 1 Hydrolyzes Fluorogenic Acyl-adenylate and Lysyl-tRNA Synthetase-generated Lysyl-adenylate. J. Biol. Chem. 282: 15137-15147 [Abstract] [Full Text]  
  • Wang, L., Zhang, Y., Li, H., Xu, Z., Santella, R. M., Weinstein, I. B. (2007). Hint1 Inhibits Growth and Activator Protein-1 Activity in Human Colon Cancer Cells. Cancer Res. 67: 4700-4708 [Abstract] [Full Text]  
  • Chou, T.-F., Wagner, C. R. (2007). Lysyl-tRNA Synthetase-generated Lysyl-Adenylate Is a Substrate for Histidine Triad Nucleotide Binding Proteins. J. Biol. Chem. 282: 4719-4727 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»