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GCF2/LRRFIP1 Represses Tumor Necrosis Factor Alpha Expression

University of Pennsylvania School of Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104

Received 30 October 2004/ Returned for modification 14 January 2005/ Accepted 30 June 2005

Tumor necrosis factor alpha (TNF-) is an important mediator of inflammation, apoptosis, and the development of secondary lymphoid structures. Multiple polymorphic microsatellites have been identified in and around the gene, and there are also multiple single-base pair biallelic polymorphisms in the introns and promoter. The TNF- –308 promoter polymorphism is a G-to-A transition which has been statistically associated with various autoimmune disorders. Some studies have found that it may directly mediate the increased transcription of TNF- in some circumstances. This study characterizes proteins interacting at the polymorphic promoter site. Affinity purification of binding proteins and confirmatory chromatin immunoprecipitation assays were used to identify the proteins. Electrophoretic mobility shift analyses and surface plasmon resonance were used to define binding characteristics. Proteins interacting at this site include GCF2/LRRFIP1 and Ets-1. GCF2/LRRFIP1 appears to act as a repressor and occupies the –308 site in cells that do not make TNF-. Cells competent to produce TNF- have Ets-1 bound to the –308 promoter site. Active transcription is accompanied by NF-B and c-Jun binding to the proximal promoter. Thus, dynamic changes on the TNF- promoter, particularly at the –308 site, accompany the transition from repressed to active transcription. GCF2/LRRFIP1 is the first TNF- repressor identified.

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