This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reeves, W. M.
Right arrow Articles by Hahn, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reeves, W. M.
Right arrow Articles by Hahn, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2005, p. 9092-9102, Vol. 25, No. 20
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.20.9092-9102.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Targets of the Gal4 Transcription Activator in Functional Transcription Complexes

Wendy M. Reeves1,2,3,{dagger} and Steven Hahn1,2*

Fred Hutchinson Cancer Research Center,1 Howard Hughes Medical Institute,2 Molecular and Cellular Biology Program, University of Washington, 1100 Fairview Ave. N., Mailstop A1-162, Seattle, Washington 981093

Received 13 May 2005/ Returned for modification 6 July 2005/ Accepted 20 July 2005

Although biochemical and genetic methods have detected many activator-transcription factor interactions, the direct functional targets of most activators remain undetermined. For this study, photo-cross-linkers positioned within the Gal4 C-terminal acidic activating region were used to identify polypeptides in close physical proximity to Gal4 during transcription activation in vitro. Of six specifically cross-linked polypeptides, three (Tra1, Taf12, and Gal11) are subunits of four complexes (SAGA, Mediator, NuA4, and TFIID) known to play a role in gene regulation. These cross-linking targets had differential effects on activation. SAGA was critical for activation by Gal4, Gal11 contributed modestly to activation, and TFIID and NuA4 were not important for activation under our conditions. Tra1, Taf12, and Gal11 have also been identified as cross-linking targets of the Gcn4 acidic central activating region. Our results demonstrate that two unrelated acidic activators converge on the same set of functional targets.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Mailstop A1-162, Seattle, WA 98109. Phone: (206) 667-5261. Fax: (206) 667-6497. E-mail: shahn{at}fhcrc.org.

{dagger} Present address: Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106.

Molecular and Cellular Biology, October 2005, p. 9092-9102, Vol. 25, No. 20
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.20.9092-9102.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kremer, S. B., Gross, D. S. (2009). SAGA and Rpd3 Chromatin Modification Complexes Dynamically Regulate Heat Shock Gene Structure and Expression. J. Biol. Chem. 284: 32914-32931 [Abstract] [Full Text]  
  • Naar, A. M., Thakur, J. K. (2009). Nuclear receptor-like transcription factors in fungi. Genes Dev. 23: 419-432 [Abstract] [Full Text]  
  • Kim, Y., Ebright, Y. W., Goodman, A. R., Reinberg, D., Ebright, R. H. (2008). Nonradioactive, ultrasensitive site-specific protein-protein photocrosslinking: interactions of {alpha}-helix 2 of TATA-binding protein with general transcription factor TFIIA and transcriptional repressor NC2. Nucleic Acids Res 36: 6143-6154 [Abstract] [Full Text]  
  • Gamper, A. M., Roeder, R. G. (2008). Multivalent Binding of p53 to the STAGA Complex Mediates Coactivator Recruitment after UV Damage. Mol. Cell. Biol. 28: 2517-2527 [Abstract] [Full Text]  
  • Kim, D.-H., Kim, G. S., Yun, C. H., Lee, Y. C. (2008). Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 913-925 [Abstract] [Full Text]  
  • Mutiu, A. I., Hoke, S. M. T., Genereaux, J., Hannam, C., MacKenzie, K., Jobin-Robitaille, O., Guzzo, J., Cote, J., Andrews, B., Haniford, D. B., Brandl, C. J. (2007). Structure/Function Analysis of the Phosphatidylinositol-3-Kinase Domain of Yeast Tra1. Genetics 177: 151-166 [Abstract] [Full Text]  
  • Baidoobonso, S. M., Guidi, B. W., Myers, L. C. (2007). Med19(Rox3) Regulates Intermodule Interactions in the Saccharomyces cerevisiae Mediator Complex. J. Biol. Chem. 282: 5551-5559 [Abstract] [Full Text]  
  • Martchenko, M., Levitin, A., Whiteway, M. (2007). Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs. Eukaryot Cell 6: 291-301 [Abstract] [Full Text]  
  • Roider, H. G., Kanhere, A., Manke, T., Vingron, M. (2007). Predicting transcription factor affinities to DNA from a biophysical model. Bioinformatics 23: 134-141 [Abstract] [Full Text]  
  • Garbett, K. A., Tripathi, M. K., Cencki, B., Layer, J. H., Weil, P. A. (2007). Yeast TFIID Serves as a Coactivator for Rap1p by Direct Protein-Protein Interaction. Mol. Cell. Biol. 27: 297-311 [Abstract] [Full Text]  
  • Taubert, S., Van Gilst, M. R., Hansen, M., Yamamoto, K. R. (2006). A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans.. Genes Dev. 20: 1137-1149 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»