This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reeves, W. M. Articles by Hahn, S. Search for Related Content PubMed PubMed Citation Articles by Reeves, W. M. Articles by Hahn, S.

Targets of the Gal4 Transcription Activator in Functional Transcription Complexes

Wendy M. Reeves^1,2,3, and Steven Hahn^1,2*

Fred Hutchinson Cancer Research Center,¹ Howard Hughes Medical Institute,² Molecular and Cellular Biology Program, University of Washington, 1100 Fairview Ave. N., Mailstop A1-162, Seattle, Washington 98109³

Received 13 May 2005/ Returned for modification 6 July 2005/ Accepted 20 July 2005

Although biochemical and genetic methods have detected many activator-transcription factor interactions, the direct functional targets of most activators remain undetermined. For this study, photo-cross-linkers positioned within the Gal4 C-terminal acidic activating region were used to identify polypeptides in close physical proximity to Gal4 during transcription activation in vitro. Of six specifically cross-linked polypeptides, three (Tra1, Taf12, and Gal11) are subunits of four complexes (SAGA, Mediator, NuA4, and TFIID) known to play a role in gene regulation. These cross-linking targets had differential effects on activation. SAGA was critical for activation by Gal4, Gal11 contributed modestly to activation, and TFIID and NuA4 were not important for activation under our conditions. Tra1, Taf12, and Gal11 have also been identified as cross-linking targets of the Gcn4 acidic central activating region. Our results demonstrate that two unrelated acidic activators converge on the same set of functional targets.

Present address: Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106.

This article has been cited by other articles:

• Gamper, A. M., Roeder, R. G. (2008). Multivalent Binding of p53 to the STAGA Complex Mediates Coactivator Recruitment after UV Damage. Mol. Cell. Biol. 28: 2517-2527 [Abstract] [Full Text]  
• Kim, D.-H., Kim, G. S., Yun, C. H., Lee, Y. C. (2008). Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 913-925 [Abstract] [Full Text]  
• Mutiu, A. I., Hoke, S. M. T., Genereaux, J., Hannam, C., MacKenzie, K., Jobin-Robitaille, O., Guzzo, J., Cote, J., Andrews, B., Haniford, D. B., Brandl, C. J. (2007). Structure/Function Analysis of the Phosphatidylinositol-3-Kinase Domain of Yeast Tra1. Genetics 177: 151-166 [Abstract] [Full Text]  
• Baidoobonso, S. M., Guidi, B. W., Myers, L. C. (2007). Med19(Rox3) Regulates Intermodule Interactions in the Saccharomyces cerevisiae Mediator Complex. J. Biol. Chem. 282: 5551-5559 [Abstract] [Full Text]  
• Martchenko, M., Levitin, A., Whiteway, M. (2007). Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs. Eukaryot Cell 6: 291-301 [Abstract] [Full Text]  
• Roider, H. G., Kanhere, A., Manke, T., Vingron, M. (2007). Predicting transcription factor affinities to DNA from a biophysical model. Bioinformatics 23: 134-141 [Abstract] [Full Text]  
• Garbett, K. A., Tripathi, M. K., Cencki, B., Layer, J. H., Weil, P. A. (2007). Yeast TFIID Serves as a Coactivator for Rap1p by Direct Protein-Protein Interaction. Mol. Cell. Biol. 27: 297-311 [Abstract] [Full Text]  
• Taubert, S., Van Gilst, M. R., Hansen, M., Yamamoto, K. R. (2006). A Mediator subunit, MDT-15, integrates regulation of fatty acid metabolism by NHR-49-dependent and -independent pathways in C. elegans.. Genes Dev. 20: 1137-1149 [Abstract] [Full Text]