This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Le Sommer, C. Articles by Hardy, S. Search for Related Content PubMed PubMed Citation Articles by Le Sommer, C. Articles by Hardy, S.

PTB Regulates the Processing of a 3'-Terminal Exon by Repressing both Splicing and Polyadenylation

UMR 6061 CNRS-Université de Rennes 1, IFR 140 Faculté de Médecine, CS 34317, 35043 Rennes Cedex, France

Received 19 May 2005/ Returned for modification 5 July 2005/ Accepted 1 August 2005

The polypyrimidine tract binding protein (PTB) has been described as a global repressor of regulated exons. To investigate PTB functions in a physiological context, we used a combination of morpholino-mediated knockdown and transgenic overexpression strategies in Xenopus laevis embryos. We show that embryonic endoderm and skin deficient in PTB displayed a switch of the -tropomyosin pre-mRNA 3' end processing to the somite-specific pattern that results from the utilization of an upstream 3'-terminal exon designed exon 9A9'. Conversely, somitic targeted overexpression of PTB resulted in the repression of the somite-specific exon 9A9' and a switch towards the nonmuscle pattern. These results validate PTB as a key physiological regulator of the 3' end processing of the -tropomyosin pre-mRNA. Moreover, using a minigene strategy in the Xenopus oocyte, we show that in addition to repressing the splicing of exon 9A9', PTB regulates the cleavage/polyadenylation of this 3'-terminal exon.

This article has been cited by other articles:

• Somberg, M., Zhao, X., Frohlich, M., Evander, M., Schwartz, S. (2008). Polypyrimidine Tract Binding Protein Induces Human Papillomavirus Type 16 Late Gene Expression by Interfering with Splicing Inhibitory Elements at the Major Late 5' Splice Site, SD3632. J. Virol. 82: 3665-3678 [Abstract] [Full Text]  
• Coutinho-Mansfield, G. C., Xue, Y., Zhang, Y., Fu, X.-D. (2007). PTB/nPTB switch: a post-transcriptional mechanism for programming neuronal differentiation. Genes Dev. 21: 1573-1577 [Full Text]  
• Sauliere, J., Sureau, A., Expert-Bezancon, A., Marie, J. (2006). The Polypyrimidine Tract Binding Protein (PTB) Represses Splicing of Exon 6B from the {beta}-Tropomyosin Pre-mRNA by Directly Interfering with the Binding of the U2AF65 Subunit. Mol. Cell. Biol. 26: 8755-8769 [Abstract] [Full Text]