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Molecular and Cellular Biology, November 2005, p. 9734-9740, Vol. 25, No. 21
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.21.9734-9740.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complex Formation of Yeast Rev1 and Rev7 Proteins: a Novel Role for the Polymerase-Associated Domain

Narottam Acharya,1 Lajos Haracska,2 Robert E. Johnson,1 Ildiko Unk,2 Satya Prakash,1 and Louise Prakash1*

Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas,1 Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary2

Received 8 July 2005/ Returned for modification 4 August 2005/ Accepted 18 August 2005

The Rev1 protein of Saccharomyces cerevisiae functions in translesion synthesis (TLS) together with DNA polymerase (Pol) {zeta}, which is comprised of the Rev3 catalytic and the Rev7 accessory subunits. Rev1, a member of the Y family of Pols, differs from other members in its high degree of specificity for incorporating a C opposite template G as well as opposite an abasic site. Although Rev1 is indispensable for Pol{zeta}-dependent TLS, its DNA synthetic activity is not required for many of the Pol{zeta}-dependent lesion bypass events. This observation has suggested a structural role for Rev1 in this process. Here we show that in yeast, Rev1 forms a stable complex with Rev7, and the two proteins copurify. Importantly, the polymerase-associated domain (PAD) of Rev1 mediates its binding to Rev7. These observations reveal a novel role for the PAD region of Rev1 in protein-protein interactions, and they raise the possibility of a similar involvement of the PAD of other Y family Pols in protein-protein interactions. We discuss the possible roles of Rev1 versus the Rev1-Rev7 complex in TLS.

* Corresponding author. Mailing address: Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Blocker Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061. Phone: (409) 747-8601. Fax: (409) 747-8608. E-mail: l.prakash{at}utmb.edu.

Molecular and Cellular Biology, November 2005, p. 9734-9740, Vol. 25, No. 21
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.21.9734-9740.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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