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Molecular and Cellular Biology, December 2005, p. 10315-10328, Vol. 25, No. 23
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.23.10315-10328.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage

Yukinori Minoshima,^1, Tetsuya Hori,^2, Masahiro Okada,² Hiroshi Kimura,³ Tokuko Haraguchi,⁴ Yasushi Hiraoka,⁴ Ying-Chun Bao,¹ Toshiyuki Kawashima,¹ Toshio Kitamura,^1* and Tatsuo Fukagawa^2*

The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan,¹ Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan,² Nuclear Function and Dynamics Unit, HMRO, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan,³ CREST of JST, Kansai Advanced Research Center, NICT, Kobe 651-2492, Japan⁴

Received 15 July 2005/ Returned for modification 30 August 2005/ Accepted 13 September 2005

We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in vertebrate cells. To determine the precise role of CENP-50, we examined its role in centromere function by generating a loss-of-function mutant in the chicken DT40 cell line. The CENP-50 knockout was not lethal; however, the growth rate of cells with this mutation was slower than that of wild-type cells. We observed that the time for CENP-50-deficient cells to complete mitosis was longer than that for wild-type cells. Centromeric localization of CENP-50 was abolished in both CENP-H- and CENP-I-deficient cells. Coimmunoprecipitation experiments revealed that CENP-50 interacted with the CENP-H/CENP-I complex in chicken DT40 cells. We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage.

Supplemental material for this article may be found at http://mcb.asm.org/.

These authors contributed equally to this work.

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