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Molecular and Cellular Biology, December 2005, p. 10675-10683, Vol. 25, No. 24
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.24.10675-10683.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Runx1 Binds Positive Transcription Elongation Factor b and Represses Transcriptional Elongation by RNA Polymerase II: Possible Mechanism of CD4 Silencing

Huimin Jiang,{dagger} Fan Zhang,{dagger} Takeshi Kurosu, and B. Matija Peterlin*

Department of Medicine, Microbiology and Immunology, Rosalind Russell Medical Research Center, University of California at San Francisco, San Francisco, California 94143-0703

Received 20 April 2005/ Returned for modification 30 June 2005/ Accepted 3 October 2005

Runx1 binds the silencer and represses CD4 transcription in immature thymocytes. In this study, we found that Runx1 inhibits P-TEFb, which contains CycT1, CycT2, or CycK and Cdk9 and stimulates transcriptional elongation by RNA polymerase II (RNAPII) in eukaryotic cells. Indeed, its inhibitory domain, spanning positions 371 to 411, not only bound CycT1 but was required for silencing CD4 transcription in vivo. Our chromatin immunoprecipitation assays revealed that Runx1 inhibits the elongation but not initiation of transcription and that RNAPII is engaged at the CD4 promoter but is unable to elongate in CD4– CD8+ thymoma cells. These results suggest that active repression by Runx1 occurs by blocking the elongation by RNAPII, which may contribute to CD4 silencing during T-cell development.

* Corresponding author. Mailing address: Box 0703, 3rd and Parnassus Ave., UCSF, San Francisco, CA 94143-0703. Phone: (415) 502-1905. Fax: (415) 502-1901. E-mail: matija{at}itsa.ucsf.edu.

{dagger} H.J. and F.Z. contributed equally to this work.

Molecular and Cellular Biology, December 2005, p. 10675-10683, Vol. 25, No. 24
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.24.10675-10683.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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