This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hertel, C. B. Articles by Korber, P. Search for Related Content PubMed PubMed Citation Articles by Hertel, C. B. Articles by Korber, P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2005, p. 10755-10767, Vol. 25, No. 24
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.24.10755-10767.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nucleosome Stability at the Yeast PHO5 and PHO8 Promoters Correlates with Differential Cofactor Requirements for Chromatin Opening

Christina Bech Hertel, Gernot Längst, Wolfram Hörz, and Philipp Korber^*

Adolf-Butenandt-Institut, Universität München, Schillerstr. 44, 80336 München, Germany

Received 8 July 2005/ Returned for modification 15 August 2005/ Accepted 5 October 2005

The coregulated PHO5 and PHO8 genes in Saccharomyces cerevisiae provide typical examples for the role of chromatin in promoter regulation. It has been a long-standing question why the cofactors Snf2 and Gcn5 are essential for full induction of PHO8 but dispensable for opening of the PHO5 promoter. We show that this discrepancy may result from different stabilities of the two promoter chromatin structures. To test this hypothesis, we used our recently established yeast extract in vitro chromatin assembly system, which generates the characteristic PHO5 promoter chromatin. Here we show that this system also assembles the native PHO8 promoter nucleosome pattern. Remarkably, the positioning information for both native patterns is specific to the yeast extract. Salt gradient dialysis or Drosophila embryo extract does not support proper nucleosome positioning unless supplemented with yeast extract. By competitive assemblies in the yeast extract system we show that the PHO8 promoter has greater nucleosome positioning power and that the properly positioned nucleosomes are more stable than those at the PHO5 promoter. Thus we provide evidence for the correlation of inherently more stable chromatin with stricter cofactor requirements.

---

* Corresponding author. Mailing address: Adolf-Butenandt-Institut, Universität München, Schillerstr. 44, 80336 München, Germany. Phone: 49-89-218075775. Fax: 49-89-218075440. E-mail: pkorber{at}lmu.de.

---

Molecular and Cellular Biology, December 2005, p. 10755-10767, Vol. 25, No. 24
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.24.10755-10767.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Wippo, C. J., Krstulovic, B. S., Ertel, F., Musladin, S., Blaschke, D., Sturzl, S., Yuan, G.-C., Horz, W., Korber, P., Barbaric, S. (2009). Differential Cofactor Requirements for Histone Eviction from Two Nucleosomes at the Yeast PHO84 Promoter Are Determined by Intrinsic Nucleosome Stability. Mol. Cell. Biol. 29: 2960-2981 [Abstract] [Full Text]  
• He, Q., Battistella, L., Morse, R. H. (2008). Mediator Requirement Downstream of Chromatin Remodeling during Transcriptional Activation of CHA1 in Yeast. J. Biol. Chem. 283: 5276-5286 [Abstract] [Full Text]  
• Barbaric, S., Luckenbach, T., Schmid, A., Blaschke, D., Horz, W., Korber, P. (2007). Redundancy of Chromatin Remodeling Pathways for the Induction of the Yeast PHO5 Promoter in Vivo. J. Biol. Chem. 282: 27610-27621 [Abstract] [Full Text]  
• Johnsson, A., Xue-Franzen, Y., Lundin, M., Wright, A. P. H. (2006). Stress-specific role of fission yeast gcn5 histone acetyltransferase in programming a subset of stress response genes.. Eukaryot Cell 5: 1337-1346 [Abstract] [Full Text]