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Molecular and Cellular Biology, December 2005, p. 10833-10841, Vol. 25, No. 24
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.24.10833-10841.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Essential WD40 Protein Cia1 Is Involved in a Late Step of Cytosolic and Nuclear Iron-Sulfur Protein Assembly

Janneke Balk,1,{dagger} Daili J. Aguilar Netz,1 Katharina Tepper,1 Antonio J. Pierik,2 and Roland Lill1*

Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Str. 6, 35033 Marburg, Germany,1 Laboratorium für Mikrobiologie, Philipps-Universität Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany2

Received 26 July 2005/ Returned for modification 19 September 2005/ Accepted 27 September 2005

The assembly of cytosolic and nuclear iron-sulfur (Fe/S) proteins in yeast is dependent on the iron-sulfur cluster assembly and export machineries in mitochondria and three recently identified extramitochondrial proteins, the P-loop NTPases Cfd1 and Nbp35 and the hydrogenase-like Nar1. However, the molecular mechanism of Fe/S protein assembly in the cytosol is far from being understood, and more components are anticipated to take part in this process. Here, we have identified and functionally characterized a novel WD40 repeat protein, designated Cia1, as an essential component required for Fe/S cluster assembly in vivo on cytosolic and nuclear, but not mitochondrial, Fe/S proteins. Surprisingly, Nbp35 and Nar1, themselves Fe/S proteins, could assemble their Fe/S clusters in the absence of Cia1, demonstrating that these components act before Cia1. Consequently, Cia1 is involved in a late step of Fe/S cluster incorporation into target proteins. Coimmunoprecipitation assays demonstrated a specific interaction between Cia1 and Nar1. In contrast to the mostly cytosolic Nar1, Cia1 is preferentially localized to the nucleus, suggesting an additional function of Cia1. Taken together, our results indicate that Cia1 is a new member of the cytosolic Fe/S protein assembly (CIA) machinery participating in a step after Nbp35 and Nar1.

* Corresponding author. Mailing address: Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Str. 6, 35033 Marburg, Germany. Phone: 49-6421-286 6449. Fax: 49-6421-286 6414. E-mail: Lill{at}mailer.uni-marburg.de.

{dagger} Present address: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.

Molecular and Cellular Biology, December 2005, p. 10833-10841, Vol. 25, No. 24
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.24.10833-10841.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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