This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiefer, H.
Right arrow Articles by Mallet, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiefer, H.
Right arrow Articles by Mallet, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2005, p. 1713-1729, Vol. 25, No. 5
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.5.1713-1729.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

ZENON, a Novel POZ Kruppel-Like DNA Binding Protein Associated with Differentiation and/or Survival of Late Postmitotic Neurons

Hélène Kiefer,{dagger} Fabienne Chatail-Hermitte,{ddagger} Philippe Ravassard, Elisa Bayard, Isabelle Brunet, and Jacques Mallet*

Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, CNRS UMR 7091, Hôpital de la Pitié-Salpêtrière, Paris, France

Received 14 May 2004/ Returned for modification 30 July 2004/ Accepted 18 November 2004

The rat tyrosine hydroxylase gene promoter contains an E-box/dyad motif and an octameric and heptameric element that may be recognized by classes of transcription factors highly expressed during nervous system development. In a one-hybrid genetic screen, we used these sites as targets to isolate cDNAs encoding new transcription factors present in the brain. We identified ZENON, a novel rat POZ protein that contains two clusters of Kruppel-like zinc fingers and that presents several features of a transcription factor. ZENON is found in nuclei following transient transfection with the cDNA. The N-terminal zinc finger cluster contains a DNA binding domain that interacts with the E box. Cotranfection experiments revealed that ZENON induces tyrosine hydroxylase promoter activity. Unlike other POZ proteins, the ZENON POZ domain is not required for either activation of transcription or self-association. In the embryonic neural tube, ZENON expression is restricted to neurons that have already achieved mitosis and are engaged in late stages of neuronal differentiation (late postmitotic neurons). ZENON neuronal expression persists in the adult brain; therefore, ZENON can be considered a marker of mature neurons. We propose that ZENON is involved in the maintenance of panneuronal features and/or in the survival of mature neurons.

* Corresponding author. Mailing address: Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, CNRS UMR 7091, BÂtiment CERVI, Hôpital de la Pitié-Salpêtrière, 83, Blvd. de l'Hôpital, 75013 Paris, France. Phone: 33 1 42 17 75 32. Fax: 33 1 42 17 75 33. E-mail: mallet{at}infobiogen.fr.

{dagger} Present address: Division of Molecular Neurobiology, Department of Basic Medical Sciences, Institute of Medical Sciences, University of Tokyo, Minato-Ku, Tokyo 108-8639, Japan.

{ddagger} Present address: Ipsogen, Luminy Biotech Enteprise, 13009 Marseille, France.

Molecular and Cellular Biology, March 2005, p. 1713-1729, Vol. 25, No. 5
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.5.1713-1729.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oikawa, Y., Matsuda, E., Nishii, T., Ishida, Y., Kawaichi, M. (2008). Down-regulation of CIBZ, a Novel Substrate of Caspase-3, Induces Apoptosis. J. Biol. Chem. 283: 14242-14247 [Abstract] [Full Text]  
  • Rozsa, F. W., Scott, K. M., Pawar, H., Samples, J. R., Wirtz, M. K., Richards, J. E. (2007). Differential Expression Profile Prioritization of Positional Candidate Glaucoma Genes: The GLC1C Locus. Arch Ophthalmol 125: 117-127 [Abstract] [Full Text]  
  • Filion, G. J. P., Zhenilo, S., Salozhin, S., Yamada, D., Prokhortchouk, E., Defossez, P.-A. (2006). A Family of Human Zinc Finger Proteins That Bind Methylated DNA and Repress Transcription. Mol. Cell. Biol. 26: 169-181 [Abstract] [Full Text]  
  • Prokhortchouk, A., Sansom, O., Selfridge, J., Caballero, I. M., Salozhin, S., Aithozhina, D., Cerchietti, L., Meng, F. G., Augenlicht, L. H., Mariadason, J. M., Hendrich, B., Melnick, A., Prokhortchouk, E., Clarke, A., Bird, A. (2006). Kaiso-Deficient Mice Show Resistance to Intestinal Cancer. Mol. Cell. Biol. 26: 199-208 [Abstract] [Full Text]  
  • Sasai, N., Matsuda, E., Sarashina, E., Ishida, Y., Kawaichi, M. (2005). Identification of a novel BTB-zinc finger transcriptional repressor, CIBZ, that interacts with CtBP corepressor. GENES CELLS 10: 871-885 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»